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Esco Labculture® Plus Class II Type A2 Biosafety Cabinet - Comlibris

Esco Labculture® Plus Class II Type A2 Biosafety Cabinet - Comlibris

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<strong>II</strong>. Biological Safety <strong>Cabinet</strong>s<br />

Table 2. Biohazard Safety <strong>Cabinet</strong> <strong>Class</strong>es<br />

<strong>Class</strong><br />

I<br />

<strong>II</strong> A1<br />

Inflow<br />

V (m/s)<br />

US:0.38<br />

EN:0.70<br />

US:0.38<br />

EN:0.40<br />

Recycle<br />

Air (%)<br />

Exhaust<br />

Air (%)<br />

5<br />

A Guide To <strong>Biosafety</strong> And <strong>Biosafety</strong> <strong>Cabinet</strong>s<br />

The terms biological safety cabinet and biosafety cabinet have been widely<br />

used to describe a variety of containment devices equipped with HEPA filter(s),<br />

designed to provide personnel or both personnel and product protection from<br />

biohazardous materials. The terms should only be applied to those devices<br />

that meet the requirements of <strong>Class</strong> I, <strong>II</strong>, or <strong>II</strong>I specifications, based on their<br />

construction, airflow velocities and patterns, and their exhaust systems.<br />

Major International Standards for <strong>Biosafety</strong> <strong>Cabinet</strong>s :<br />

� American Standard NSF 49:2002<br />

� European Standard EN 12469:2000<br />

� Australian Standard AS 2252<br />

�<br />

Japanese Standard JIS K 3800:2000<br />

<strong>II</strong> <strong>A2</strong><br />

<strong>II</strong> B1<br />

<strong>II</strong> B2<br />

<strong>II</strong>I<br />

US:0.50<br />

EN:0.40<br />

US:0.50<br />

EN:0.40<br />

US:0.50<br />

EN:NA<br />

Closed:<br />

>50”WC<br />

Control Plenum<br />

Surrounded by<br />

0 100 Outside Air<br />

70 30 Outside Air<br />

70 30 Negative Plenum<br />

30 70 Negative Plenum<br />

0 100 Negative Plenum<br />

0 100 Negative Plenum<br />

Exhaust<br />

Alternatives<br />

Inside room /<br />

Hard duct<br />

Inside room /<br />

Thimble duct<br />

Inside room /<br />

Thimble duct<br />

Hard duct<br />

only<br />

Hard duct<br />

only<br />

Inside room /<br />

Hard duct<br />

<strong>Biosafety</strong><br />

Level<br />

1, 2 & 3<br />

1, 2 & 3<br />

1, 2 & 3<br />

1, 2 & 3<br />

1, 2 & 3<br />

1, 2, 3 & 4<br />

Source: Laboratory <strong>Biosafety</strong> Manual. Second Edition (revised). Interim guidelines. World Health Organization. p32.

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