DNA Plant HTS 96 Kit

Scheme Invisorb ® DNA Plant HTS 96 Kit/VPlease read protocols prior the start of the preparation○ transfer up to 50 mg plant material into the Qiawell plate andhomogenize with Mixer Mill MM 300 (Retsch) under liquid N 2.○ add 400 µl Lysis Buffer P Mix (inclusive Proteinase K and RNase A)to each well of the Qiawell plate l○ Incubate at 65 °C for 60 min,○ centrifuge for 10 min at 1.700 x g (4.000 rpm)○○ Place the 1 ml Collection Plate in the base of the Invisorb ® 96Vacuum Manifold○ Transfer 350 µl Lysate carefully from Qiawell plate into the wellsfrom the Prefilter Plate.○ Switch on vacuum source○○○add 350 µl Binding Buffer P to each well, mix completelyplace a waste tray in the base of the manifold, add the topplateplace the DNA Binding Plate C on the top of the manifold○Transfer the probes from 1 ml Collection Plate to the DNA BindingPlate C.○○○○○○○add 500 µl Wash Buffer I to each wellapply vacuum for 3 min or until all wash buffer was runningthroughadd 700 µl Wash Buffer II to each well of DNA BindingPlate Dapply vacuum for 3 minRepeat the wash stepremove all waste from the waste trayapply vacuum for 20 min to dry the membrane○ place the spacer in the manifold○ place than1 ml Collection Plate inside the base of themanifold, add the top plate○ place the DNA Binding Plate D on the top of the manifold○ add 60 µl Elution Buffer D per cavity○ incubate 5 min at RT○ apply vacuum for 52 min13Invisorb ® Plant DNA HTS 96 Kit /V and C 1010