DNA Plant HTS 96 Kit

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ContentsKit content of Invisorb ® DNA Plant HTS 96 Kit/ C 3Kit content of Invisorb ® DNA Plant HTS 96 Kit V 4Safety information 5Product characteristic of the Invisorb ® DNA Plant HTS 96 Kit 6Yield and quality of genomic DNA 6Important points before starting a protocol 7Sampling and storage of starting material 7Preparing reagents and buffers 7Reagents and equipment to be supplied by user 7Principle and procedure 8Scheme of Invisorb ® DNA Plant HTS 96 Kit/ C 9Protocol: DNA extraction from plant material for use on a centrifuge 10Scheme of Invisorb ® DNA Plant HTS 96 Kit/ V 13Protocol : DNA extraction from plant material for use on aInvisorb ® 96 Vacuum Manifold 14Invisorb ® 96 Vacuum Manifold 15Troubleshooting 16Appendix 17Ordering information 18Invitek distributors 192Invisorb ® Plant DNA HTS 96 Kit /V and C 1010
Kit contents of Invisorb ® DNA Plant HTS 96 Kit/ CStore lyophilized Proteinase K at 2 - 8 °C. Store d iluted Proteinase K at -20 °C, but repeatedfreezing and thawing will reduced the activity dramatically.Dividing the Proteinase K into aliquots and storage at -20°C is recommended.Store all other kit components at room temperature (RT)!2 x 96 DNA extractions 4 x 96 DNA extractions 24 x 96 DNA extractionsCatalog No. 7037300200 7037300300 7037300400Lysis Buffer P 2 x 40 ml 4 x 40 ml 24 x 40 mlBinding Buffer P 80 ml 160 ml 900 mlElution Buffer D 30 ml 2 x 30 ml 350 mlProteinase K 2 x 2 ml 4 x 2 ml 24 x 2 mlRNase A 2 x 4 ml 4 x 4 ml 24 x 4 mlWash Buffer I75 ml(final volume 150 ml)2 x 45 ml(final volume 2 x 150 ml)2 x 75 ml(final volume 2 x 150 ml)4 x 45 ml(final volume 4 x 150 ml)2 x 350 ml(final volume 700 ml)4 x 270 ml(final volume 900 ml)Wash Buffer IICollection Plate; 2 ml 4 2 x 4 12 x 4Collection Plate, 1 ml 2 4 6 x 4Prefilter Plate 2 2 x 2 6 x 4DNA Binding Plate C 2 4 6 x 4Plate Lid 4 8 48Sealing Foils 2 4 24Manual 1 1 1Initial steps Add 75 ml 96-100%ethanol to the bottle WashBuffer IAdd 105 ml of 96-100%ethanol to each bottleWash Buffer II, mix shortlyand keep the bottle alwaysfirmly closed!Add 2 ml ddH 2 O to eachtube Proteinase K, mixthoroughly and store at-20°C !Add 75 ml 96-100%ethanol to each bottleWash Buffer IAdd 105 ml of 96-100%ethanol to each bottleWash Buffer II, mix shortlyand keep the bottle alwaysfirmly closed!Add 2 ml ddH 2 O to eachtube Proteinase K, mixthoroughly and store at -20°C !Add 350 ml 96-100%ethanol to each bottleWash Buffer IAdd 630 ml of 96-100%ethanol to each bottleWash Buffer II, mix shortlyand keep the bottle alwaysfirmly closed!Add 2 ml ddH 2 O to eachtube Proteinase K, mixthoroughly and store at-20°C !3Invisorb ® Plant DNA HTS 96 Kit /V and C 1010
Page 5 and 6: Safety InformationWhen working with
Page 7 and 8: Important points before starting a
Page 9 and 10: Scheme Invisorb ® DNA Plant HTS 96
Page 11 and 12: 3. Binding of the genomic DNA to th
Page 13 and 14: Scheme Invisorb ® DNA Plant HTS 96
Page 15 and 16: 6. Removing of ethanolPlace the was
Page 17 and 18: AppendixGeneral notes on handling D
Page 19 and 20: Invitek DistributorsAustraliaMedica

DNA Plant HTS 96 Kit

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