DNA Plant HTS 96 Kit

Scheme Invisorb ® DNA Plant HTS 96 Kit/ CPlease read protocols prior the start of the preparation○○○○50 mg plant material into the QIAwell plate,homogenize with MixerMill MM 300 (Retsch) under liquid Nadd 400 µl Lysis Buffer P Mix (inclusive Proteinase K and RNaseA) to each well of the Qiawell plateIncubate at 65 °C for 60 min,centrifuge for 10 min at 1.700 x g (4.000 rpm)○○○○○transfer 350 µl of the lysate carefully from the QIAwell plate intothe wells of the Prefilter Plate.close the Prefilter Plate with a Plate Lidload the whole block (Prefilter Plate/ 2 ml Collection Plate) into theholder and place the whole assembly in the rotor bucket.centrifuge at 1.700 x g (4.000 rpm) for 5 mindiscard the Prefilter Plate○○○○○○add 350 µl Binding Buffer P to each well of the 2 ml CollectionPlateplace the DNA Binding Plate C on top of a new 2 ml Collection Plate.Transfer the suspension completely into each well of the DNABinding Plate C.Close the DNA Binding Plate C with the Plate Lid and incubate for1 min at RTCentrifuge at 1.700 x g (4.000 rpm) for 10 min at RTRemove the Plate Lid and discard the filtrate.○○○add 500 µl Wash Buffer I to each well of DNA Binding Plate Dcentrifuge at 1.400 - 1.700 x g (3.700 – 4.000 rpm) for 5 min at RTRemove the Plate Lid and discard the filtrate○○○○○○○add 700 µl Wash Buffer II to each well of DNA Binding Plate Ccentrifuge at 1.700 x g (4.000 rpm) for 5 min at RTdiscard filtrate and place the DNA Binding Plate C back on the topof a 2 ml Collection PlateRepeat the wash stepremove all waste from the waste traycentrifuge at maximum speed for 15 min to dry the membranediscard 2 ml Collection Plate○○○○○○○place the DNA Binding Plate C on the top of a 1 ml CollectionPlate or of a Microtube Plateadd 60 µl Elution Buffer Dincubate 5 min at RT·centrifuge at 1.700 x g (4.000 rpm) for 5 minRepeat the elution stepTake the DNA-Binding Plate C and the 1 ml Collection Plate out ofthe centrifugeDiscard the DNA-Binding Plate C9Invisorb ® Plant DNA HTS 96 Kit /V and C 1010