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ChromJournalInnoVAtIVe pRoducts foR chRomAtoGRAphyNew from Pall Life Sciences –certified,low extractable syringe filters for LC/MSLiquid Chromatography/Mass Spectrometry(LC/MS) is a powerfulanalytical analysistechnique used forvarious applications.It is often used fordetection of a broadrange of analytes,structural elucidation,polymer characterisation,pharmacokineticsstudies, proteomics, andquantitative analyses.Extraction and leachingof chemical compoundsfrom filters are majorconcerns in analyticalsample preparations,particularly whenusing chromatographictechniques interfacedwith MS detectorsfor analysis. Theseextractable andleachable materials,when not controlled,may jeopardise analyticalresults and datainterpretation.Pall Life Sciences is proudto present a new uniqueAcrodisc ® MS syringe filterwith WWPTFE membrane.This Acrodisc ® MS syringefilter has extremely lowlevels of extractables andis certified for LC/MS. Thefollowing extractablesstudy demonstrates whythe Acrodisc ® MS syringefilter is ideal for LC/MSsample preparation andhow it’s performanceaids in obtaining accurateexperimental results.the WWptfe membranePall life Sciences WWPtFe membraneis an optimised water-wettablepolytetrafluoroethylene membrane which offersextremely low extractable levels with good flowrates and retention efficiency. The WWPTFEmembrane also provides excellent chemicalcompatibility with both aqueous and mildorganic solvents.extractables studythe cleanliness of three different types ofsyringe filters - Pall’s Acrodisc ® MS syringe filter,and two alternative syringe filters - is evaluatedbased on the level of extractables in the filtereffluents using LC/MS under the same filtrationand analytical conditions. Ten filters from onelot of product are tested for each of the threefilter types.IntensityIntensityRetention time (Minutes)D, Hydrophilic PtFeC, PVDFB, acrodisc MSa, Controlfigure 1. overlaid tIC chromatograms usingacetonitrile:water (50:50, v/v) as the testfluid. (A - orange) Control, (B - green) Effluentfrom Pall’s acrodisc ® MS syringe filter, (C -purple) alternative PVDF filter, and (D - black)alternative hydrophilic PTFE filter.D, Hydrophilic PtFeC, PVDFB, acrodisc MSa, Controlthe analytical procedure for this evaluationconsists of an UPLC (Ultra Performance LiquidChromatography) gradient elution methodcapable of detecting a broad range of organiccompounds in the eS+ (electrospray positive)MS ionisation mode. Pure acetonitrile, 50%acetonitrile in water, pure methanol, and 50%Methanol in water are the four test fluidsbased on the most commonly used solvents foranalytical sample preparations.Representative chromatograms are provided inFigures 1 – 4In Figures 1 and 2, it is observed that theAcetonitrile:Water (50:50, v/v) mixture tends toshow more extractable peaks in the hydrophilicPTFE and PVDF filters than pure Acetonitrile.It is also noticed that the performance of theacrodisc ® MS syringe filter is more consistentregardless of the strength of the acetonitrilecontaining solvent.IntensityIntensityD, Hydrophilic PtFeC, PVDFB, acrodisc MSa, ControlRetention time (Minutes)figure 2. overlaid tIC chromatograms usingpure acetonitrile as the test fluid.(A - orange) Control, (B - green) Effluent fromPall’s acrodisc ® MS syringe filter, (C - purple)alternative PVDF filter, and (D - black)D, Hydrophilic PtFeC, PVDFB, acrodisc MSa, ControlRetention time (Minutes)Retention time (Minutes)figure 3. overlaid tIC chromatograms usingMethanol:Water (50:50, v/v) as the test fluid.(A - orange) Control, (B - green) Effluent fromPall’s acrodisc ® MS syringe filter,(C - purple) Alternative PVDF filter, and(D - black) Alternative hydrophilic PTFE filter.figure 4. overlaid tIC chromatograms usingMethanol as the test fluid. (A - orange)Control, (B - green) Effluent from Pall’sacrodisc ® MS syringe filter, (C – purple)Alternative PVDF filter, and (D - black)Alternative hydrophilic PTFE filter.14VWR InteRnatIonal I chromJournal Issue 12 I 2012