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ChromJournalInnoVAtIVe pRoducts foR chRomAtoGRAphythe new VWR evaporative lightscattering detector (elsd)Introduction:the new VWR elSD 90 with theVWR-Hitachi ChromasterAdvantages of elsd:• nearly Universal (ideal for Unexpected analyteDetection).• Compatible with gradients• Mass Response Detector• Simple and robust (ideal for methoddevelopment)examples of applications where theVWR elSD 90 is particularly suited are:• Carbohydrates (mono, di and oligosaccharides)and polyols obtained with a gradient elution.• Polar and non polar lipids, a very convenientand efficient alternative to GC analyses• amino acids without any tedious derivationstep before detection• Water and fat soluble vitamins analysedsimultaneously• Inorganic ions without the use of anyadditional equipment such as ion suppressors• organic and phenolic acidsColumn temperature: 40 °Celuent a: Watereluent B: acetonitrileIsocratic: 28% A/72% B, eluent &degassed mixed by the pumpFlow rate: 1.0 ml/min, pressure: 79 barInjection volume: 20 µlStop time: 15 minelSD conditions: Drift tubetemperature: 50 ºC, n2 gas pressure:3.5 bar, gain 12,filter 6sAmong the detectors available in LiquidChromatography, the evaporative light-Scattering Detector (elSD) has become, in recentyears, a well established instrument thanks toseveral theoretical studies based on fundamentalinvestigations and numerous applicationsprovided during the last thirty years.elSD is considered as an almost universal,powerful and cost effective detection technique,and is ideally suited to the majority of liquidchromatography applications. today the powerof this detection mode is further extended witha new model which uses a genuine and efficientlow-temperature technology combined withan innovative detection chamber, providing thehighest sensitivity with all compounds includingthose that are semi-volatile and thermo-labile.Descriptionanother great advantage with elSD is that allnon volatile analytes without a chromophore,can be detected as the elSD is a mass responsedetector.to show the improvements with the new VWR-Hitachi Chromaster HPlC system, we presentsome specific examples as well as benefits ofusing the Chromaster with the VWR elSD 90.the chromatograms in Figures 1 and 2 used thefollowing systems set-up;Column:DescriptionShodex AsahipakNH2P-50 4EVWr-Hitachi Chromaster system configurationCat. No.Chromaster 5110 pump with AP valve with auto purge valve, washing mechanism, e-Line cable (0.5 m) 903-05016 channel Degassing unit (built-in accessory for 5110 pump) 4 channels for solvents, 2 channels for AS washingsolution, 480 µl/flow path903-0503700 µl (static mixer), for conventional (0.4 - 1.8 ml/min) use. Standard accessory for LPG Unit for 5110 903-0506Low-pressure Gradient Unit for 5110 (built-in accessory for 5110 pump), proportioning valve, conventional mixer, tubes 903-0502Chromaster 5210 Autosampler Sample rack (1.5 mlx120), 175 µl syringe (0.1 to 100 µl) e-Line cable (0.8 m). 903-0509Chromaster 5310 Column Oven e-Line cable (0.5 m) is equipped as standard accessory. Power unit is built-in. 30 cmcolumn x 3 pcs can be loaded.903-0520Chromaster Organizer Cabinet for solvent bottles. Power supply function for Pump, AS, IFC Board, Detectors 903-0537GUI Controller for Chromaster 5000 series Interface Control Board 903-0546Evaporative Light Scattering Detector 90 903-0267Poresize(Å)Particlesize(µm)I-øxL(mm)Cat. No.100 5 250x4.6 554-088016VWR InteRnatIonal I chromJournal Issue 12 I 2012