1. Introduction
1. Introduction
1. Introduction
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Study on capabiliry of producing enzymes and'.. 221<br />
After comparing srith 165 rDNAs which have published in gene bank we realized<br />
that the similarity between TL53 and Banillus subtilis is99,7%o. The phylogenetic tree<br />
was constructed based on 165r DNA of TL53 strain and related species of genera<br />
Bacillus.<br />
The results showed that the TL53 strain was located at the Bacillus subtilis<br />
clade.<br />
After comparing the morphological, physioloeical characteristics of TL53 with<br />
Bacillus subtilis we can confirm that TL53 strain is Bocillus subtilis.<br />
t1l<br />
121<br />
t3l<br />
t4l<br />
REFERENCF^S<br />
Asubel, F. M., Brent, R., Kingston, R.E., Mmre- D-D-, S€idman, J.G., Smith, J.A. and<br />
Struhl, K. (1999). Short protocols in molecular Hog5. 4th Ed. John Weiley & Sons, Inc.<br />
pp 13-50.<br />
John G.H., NoeI R.K., Peter H.A.S., James T*S., Stsxlef, T.\tr. (1994). Bergeys Manual of<br />
Detertninatiue Bacteriology, ninth Edition- Wiliiam r&mlhn+ pp 559'562.<br />
Phillipp Gerhardt (1994), Methods for General and Molenrler Bacteriolory, Am.erican<br />
Society for Microbiology, Washington, DC. pp 622$2tl-<br />
Reissig J. L., Strominger J.L. , aud kloi,r LF. (1955). A modifrnntiou colorimetric<br />
method for the estimation of N-acetylqmino sugarst J. BioI"CIIart g?35S9<br />
TAp CHi KHOAHgCDHQGHN, KHTN &CN, T.XXll, S53CPT., ffi<br />
NGHTEN CIIU KHA NANC SINH ENZYM VA CAC CNAT XNEXC<br />
srNH cria cHriNc vI KHUdN TLsB PHAN lAp ru cAc rvrAu oAt<br />
rHU DuoC ruwoN qu6c GIA cAr BA<br />
DAo Thi Lrldng(r), PhAm Dric Nggc(2)<br />
'"Tfung td,m C6ng nghQ Sinh hoc, Eq.i hpc Qudc gia Hd, NQi<br />
tuKhrlo Srnft ftoc, Trting Dq,i hgc Khoa h7c Tg nhiin, Dg,i hqc Qudc gia Hd Nfli<br />
TL5B Ie eh*oe ri khudn dtigc ph6n lap trl c6c mdu tldt & Vrrdn Qudc gia Cet Ba,<br />
thinh phdHii Phdng. Chring ndy c6 ho4t tfnh enzym m4nh phAn giii c6c hop chdt htu<br />
co nhrt tirrh bdc- prmen- Iipit, kitin, celluloz vh c6 hoat tfnh m4nh kh6ng c6c chtrng vi<br />
sinh vAt kidm r'?ir"t: Chu:g niy drrgc x6c dinh thu6c chi Bacillus dga tr6n c6c dac didm<br />
hinh thAi, sinh F rA &de tr€n r.i6c phAn tich trinh tg gen md h6a ARNr 165 thi chring<br />
ndy c6 m6i quan he cL,@ Icel ph.6t sinh gdn giri nhdt ddi v6i lodi Bacillus subtilis. Do<br />
c6 nhi6:u dq.c didm rrr. Tl€t c4..me nAy co thd dtrgc xem x6t tld srl dung trong chd tqo chd<br />
phdm probiotic dtne rrEEE =.:rdu" tir'g thtl' sAn.
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