Enzymes lab turn-in worksheet

Question 4: Describe in words what the graph of your data suggested to you about invertase and how temperature influences howfast it works.Question 5: Does your data support or refute your hypothesis? Why or why not?Question 6: Copy the data from your lab notebook, and add it here! (There should be BOTH a table and a graph pasted here.)Experiment 2: How will pH influence invertase activity?Question 7: Write down your hypothesis related to how changing the pH of the reaction solution will influence how fast invertaseworks (i.e. will more acidic, neutral or basic pHs help invertase work the best, or will there be no difference observed). Keep inmind that your hypothesis does not need to be correct, but it does need to be clearly explained.Question 8: Write down how your method for testing how pH influences the speed with which invertase breaks down sucrose. Bespecific enough so that someone reading your method would be able to carry out the same steps you will follow.Page 2 of 4

Question 9: Describe in words what the graph of your data told you about invertase and how pH influences how fast it works.Question 10: Does your data support or refute your hypothesis? Why or why not?Question 11: Copy the data from your lab notebook, and add here! (There should be a table and graph pasted here)Experiment 3: How will [S] influence invertase activity?Question 12: Write down how you think changing *S+ will influence how fast invertase works in the form of a testable hypothesis.As before, your hypothesis does not need to be correct, but it does need to be clearly explained.Question 13: Write down how you will carry out your experiment to test how *S+ influences the speed at which invertase breaksdown sucrose. Be specific enough so that someone reading your method would be able to carry out the same steps you willfollow.Page 3 of 4

Question 14: Describe in words what the graph of your data told you about invertaseand how *S+ influences how fast it works.Question 15: Does your data support or refute your hypothesis? Why or why not?Question 16: Copy the data from your lab notebook, and add here! (There should be a table and graph pasted here.) Be sure to alsothe lab summary questions (17-19) below!Lab Summary:Question 17: Which factors (temperature, pH and/or *S+) influences the speed at which invertase breaks down sucrose?Question 18: Which factors (temperature, pH and/or *S+) do you think influences how well invertase was able to reduce theactivation energy for this reaction? Explain your answer.Question 19: Of those factors that did influence invertase's speed in breaking down sucrose, were there any differences in how theyinfluences invertase? Provide an explanation for your findings.I hope you enjoyed the lab!Be sure to turn in your word processor file to the instructor via the Work2Do tab.Page 4 of 4