AAPPTec Focus XC Series - Fully Automated Peptide ... - ACS

Focus XC Series
Fully Automated Peptide Synthesizers
Focus XC I Economy
Focus XC II DNA/PNA
Focus XC-(2),(4),(6) Research
Focus XC III Production
APPT1091 FocusXC Brochure_REV.indd 1 5/23/11 4:47 PM

FOCUS XC SERIES
AAPPTec’s Focus XC series of instruments are easy to use, fully
automated solid phase peptide synthesizers. 1 The Focus XC is
capable of simultaneous and parallel peptide synthesis. The powerful
combination of utilizing 1 to 6 reactors, 24 amino acids, up to 8 solvent/
reagent bottles, and versatile user-friendly software gives the Focus XC
unique abilities. Novice and expert chemists have virtually unlimited
synthesis options. 2 The Focus XC is specially designed for Fmoc and Boc
chemistries, but has the capability to be used for many other chemistries
(DNA, PNA, RNA). The Focus XC series is an excellent choice for
discovery research, synthetic methods, process development, and
production for phase I/II/III clinical trials.
Regardless of your research, the Focus XC is guaranteed to increase your
laboratory productivity.
Additional benefits of the Focus XC instruments:
• The solution for difficult peptide sequences
• The highest crude purity
• The widest scale range on the market
• Walk away, 24/7 worry-free operation
• Reliable, provides years of successful syntheses
• Quiet operation
• Robust, low maintenance
• Low operating costs
• Flexible, sophisticated software
• Reaction monitoring with system alerts
• Access to AAPPTec’s world class support.
With the Focus XC, AAPPTec advances its already successful line of
bench-top peptide synthesizers, which started in 1990 when the Model
90 revolutionized the peptide industry. After more than 20 years of
proven operation and continued improvements, AAPPTec’s bench-top
peptide synthesizers have become an industry standard for peptide
research and production.
1
Johnson & Johnson - “Very easy (dummy proof)
software, great RV-mixing, robust, easy to clean,
better than ABI, very good peptides, really likes
the scale up ability.”
2
Vanderbilt University - “User friendly, walk away
synthesizer, very easy software.”
ACP (65-74)
Crude Peptide
HEATING OPTION
Moderate heating during coupling has proven to be very beneficial for
the synthesis of some peptides. Recently, conventional heating was
shown to be as effective as microwaves in peptide synthesis. 3 The Focus
XC has a heating option that allows the reaction vessel to be heated
during reactions: the heating module applies heat only during the steps
specified by the user thus eliminating unnecessary heating that can
damage heat sensitive sequences or sequence position.
3 Bacsa B, Horváti K, Bõsze S, Andreae F, Kappe CO J. Org. Chem. 2008, 73, 7532-42.
SONICATION OPTION
Untrasound has been shown to accelerate reactions and can also yield
products not obtainable by purely thermal reactions. 4 Sonication has
proven to disrupt aggregation and accelerate diffusion of reagents into
the resin beads thus speeding up coupling reactions; particularly difficult
couplings. AAPPTec offers an optional sonication module for the Focus
XC which applies ultrasound to the reactions vessels at user-specified
intervals during the syntheses.
4 Hickenboth CR, et al. Nature, 2007, 446, 423-7.
UV MONITORING OPTION
The UV monitoring option identifies difficult
coupling positions in real-time and makes
predefined adjustments to the synthesis
protocols to optimize coupling efficiency.
This minimizes deletion peptides that form
when deprotection reactions are slow and
the intermediate peptide-resin is not fully
deprotected before couplings – no more
failed syntheses due to unexpected slow
deprotections.
2000
1600
1200
800
400
1.8
1.6
1.4
1.2
1.0
0.8
0.6
0.4
0.2
Optional Sonication/
Heating Module
1-ALA 2-VAL 3-ILE 4-PHE 5-PHE
Real-Time Feedback of the
UV Monitoring System
DEHFVANDTRTKKRLAVEKKAELKIGRLNK
0
0 5 10 15 20 25 30 35
Crude Peptide
APPT1091 FocusXC Brochure_REV.indd 2-3 5/23/11 4:47 PM

FOUR CONFIGURATIONS
The Focus XC series has four different configurations providing options
for different scales from research to production, different numbers of
peptides produced, and affordable options.
Focus XC I • Economy
The Focus XC I is a fully-automated economical synthesizer that can
accommodate a user’s needs and budget. The Focus XC I is the ideal
instrument for any research laboratory in need of a dependable, singlereactor
peptide synthesizer with a large scale range.
• 1 Reactor (25 – 100mL)
• 5 Solvent/Reagent Lines (variable bottle sizes)
• 16 Amino Acid Containers (90mL)
Options include additional reactors, additional solvent/reagent lines,
additional amino acid containers, heating and sonication modules, and
UV detection.
Focus XC II • DNA/PNA
The Focus XC II was created to meet the demands of continuous medical
research advancement. The Focus XC II is specially designed to deliver
volumes as low as 100µL to support PNA, DNA, and RNA synthesis. The
Focus XC II can also be used for traditional solid phase peptide chemistry
to produce small numbers of high quality peptides.
• 2 Reactors (2 – 25mL)
• 6 Solvent/Reagent Lines (variable bottles sizes)
• 24 Monomer Containers (10 – 30mL)
Focus XC2, Focus XC4 and Focus XC6 • Research Instruments
The Focus XC(2-6) research scale instruments are designed to efficiently
synthesize 1 to 6 high quality peptides in parallel. These instruments
can prepare peptides in a scale range of 0.05 to 45 mmol depending
on the reactors configuration and size. The Focus XC instruments are
ideal for any research laboratory where efficiency, reliability, and high
quality peptide products are important. The Focus XC instruments
are invaluable tools for discovery research, synthetic methods, process
development and even production for phase I and phase II clinical trials.
• 2 – 6 Reactors (25, 50, 100, 200, or 300mL)
• 6 Solvent/Reagent Lines (variable bottle sizes)
• 24 Amino Acid Containers (90, or 250mL)
• Measuring and Preactivation Vessel (15, 45, or 90mL)
Options include additional solvent/reagent lines, additional and/or
larger amino acid containers, larger measuring and preactivation vessels,
heating and sonication modules, and UV detection.
Focus XC III • Production
The Focus XC III is a fully-automated production scale synthesizer small
enough to sit on a standard bench top. This instrument is capable of
preparing hundreds of grams of peptide in a single synthesis. The Focus
XC III is the perfect instrument for preparing peptides for cGMP and
small scale production.
• 1 Reactor (500mL, 1, 2, 3, or 5L)
• 6 Solvent/Reagent Lines (variable container sizes)
• 4 Amino Acid Containers (variable amino acid sizes)
Options include additional solvent/reagent lines, 8 additional amino acid
containers, heating and cooling reactors, and UV detection.
Focus XC II
Focus XC with 2 Reactors
Focus XC with 1L Reactor
REACTION VESSEL DESIGN
The Focus XC series uses interchangeable reactors in sizes ranging from
5mL to 5L. The reactors are made of glass for clear observation of the
synthesis and easy access to the resin for manual additions or sampling.
A durable glass frit ensure that the resin remains intact and in the reactor.
VARIABLE SYNTHESIS SCALE
The Focus XC series uses interchangeable reactors in sizes from 5 ml
up to 5 L reactors depending on the Focus configuration. The reaction
vessels are made of glass for ease of use and observation of the
synthesis.
MIXING
The Focus XC series utilizes variable speed wrist-action mixing, nitrogen
bubbling, or a combination of both.
EFFICIENT RESIN WASHING
The unique design of the reaction vessel allows the resin to be washed
from the top of the reactor, through the resin, and then to the waste.
The shower head spray feature efficiently and completely washes the
sides of the reactors from the neck down, ensuring that the resin remains
in the bottom of the reactor so that each bead makes uniform contact
with the wash solvent. As an added feature the user to can also choose
to wash the resin by delivering solvent from the bottom of the reactor.
SOLVENT/REAGENT BOTTLES
Configurations of solvent and reagent vary throughout the Focus XC
models. The user has the option of customizing the configuration they
receive.
AMINO ACID/REAGENT CONTAINERS
The Focus XC is delivered with 16, 24, or 36 amino acid/reagent
containers. Each amino acid container can be assigned to a single
residue in the peptide sequence or can be used as stock amino acid
reservoirs and accessed multiple times for multiple couplings for multiple
peptides. The Focus XC software allows the user to assign specific
amino acids (or reagents) to each amino acid container as needed for the
synthesis. Furthermore, if a particular peptide requires excess volume of
any amino acid it can be assigned to more than one vial. This flexibility
allows the Focus XC to complete an entire synthesis unattended,
regardless of scale or peptide length.
The Focus XC can utilize either powder or pre-dissolved amino acids.
The amino acid solution can either be preactivated within the amino acid
vial or a portion of the stock solution can be transferred to the measuring
vessel for preactivation. All liquids in the Focus XC are transferred under
nitrogen pressure from a solvent or reagent container. This creates a
completely inert atmosphere that is maintained by nitrogen or argon.
Optional Reactor Sizes
Transfers from AA to Single RV
Transfers from AA to Multiple RVs
Double Coupling from One AA
Double Coupling to Two RVs
APPT1091 FocusXC Brochure_REV.indd 4-5 5/23/11 4:47 PM

PURPOSEFUL, EASY-TO-USE SOFTWARE
The Focus XC software comes with preloaded Boc and Fmoc protocols
that allow any user to prepare high quality peptides. On-screen
instructions direct users through every step of the synthesis process
and point-and-click commands make operation simple. 5 Experienced
users can quickly create custom protocols, for instance, special coupling
protocols or alternative deprotection methods. The flexibility and the
versatility of the software make the Focus XC series the ultimate peptide
synthesizers.
5
University of Nebraska - “Easy to use, great flexibility, great for scale up and long peptides,
no drawbacks.”
RELIABILITY
The Focus XC is highly reliable and utilizes proven technology. With
simple preventative maintenance by the user, the Focus XC will provide
reliable and continuous service for many years. The instruments are very
easy to troubleshoot and the majority of repairs can be done by the user
if needed: thus reducing possible downtime of the instrument, saving
you both time and money.
TECHNICAL SUPPORT
All of AAPPTec’s instruments are backed by our unparalleled expertise
in peptide chemistry. An experienced and qualified team of technical
support personnel, engineers, and application chemists is dedicated to
answering all of your question related to peptide chemistry, instrument
operations, or instrument applications. 6
6
Research Triangle International - “Speeds up my work, simple to
use, very good customer service. I like the Focus XC.”
ON-INSTRUMENT CLEAVAGE
With on-instrument cleavage, the Focus XC extends the convenience,
time savings and other productivity advantages of automation to the
process of removing the product from the resin. The Focus XC can
cleave products from resin using any of the common cleavage protocols
except HF based cleavage protocols. Cleavage with TFA, TFMSA,
TMSOTf or specialized cleavage reagents can be easily performed on
the Focus XC without having to transfer the resin from the reaction
vessel. All scavengers and additives utilized in common cleavage
cocktails are compatible with the Focus XC. Just prepare the cleavage
mixture and place it in one of the amino acid/reagent vials and the
Focus XC can transfer it to the selected reaction vessel. The Focus XC
will automatically agitate the mixture for a length of time specified by
the user, then transfer the solution containing the cleaved product to
different container.
The solution containing the cleaved product can be transferred by any
one of three ways:
• It can be collected in a designated amino acid/reagent container.
• It can be transferred to another reaction vessel for further elaboration.
• It can be sent to a flask or other container through one of the “waste”
lines. The waste line can be flushed prior to cleavage or one of the
waste lines can be dedicated for products only.
Product cleavage on the Focus XC is easy and convenient and without
the losses encountered in transferring product resin from a reaction
vessel to a cleavage vessel. The Focus XC, with its flexibility, ease of use,
multiple simultaneous synthesis capability, and convenient on-instrument
cleavage, is a productivity enhancing tool suitable for any laboratory.
Three Ways to Transfer
Cleavage Product Solution
1. Transfers from RV to AA
2. Transfers from RV to RV
3. Transfers from RV to Flask
APPT1091 FocusXC Brochure_REV.indd 6-7 5/23/11 4:47 PM

FEATURES
• Fully automated, walk away synthesizer with on-instrument
cleavage capabilities
• Reliable performance with low maintenance
• Affordable, low operating and maintenance costs
• Flexibility for any chemistry (peptide, DNA, PNA, RNA, organic,
or combinatorial)
• Applications in research, process development, and production
• Parallel syntheses with wide variability in scale
• Intuitive software for standard and custom protocols
• Compact design, small footprint, no hood needed
SPECIFICATIONS
Width: 27.5” (670 cm)
Depth: 15” (38.5cm)
Height: 32” (81cm)
Weight: 85 lb (38.63 kilograms)
Fluid Transfer: Nitrogen pressure regulated to 10 psi (Pa 68947)
Control: The Focus XC is controlled by a windows based PC. The
CPU and associated hardware (mouse, keyboard, monitor, printer, and
standard communication ports) are compatible with the control software.
STANDARD CONFIGURATIONS
• XC I – 1 x 50mL RV (25, 100mL optional), 16 x 90mL amino acid
containers (250mL optional), 2 x 2L solvent bottles, 2 x 0.5L reagent
bottles, 1 x 1L deprotection solution bottle
• XC II – 2 x 2mL RVs (5, 10, 25mL optional), 24 x 10mL amino acid
containers, 6 solvent/reagent bottles (variable sizes)
• XC2 – 2 x 50mL RVs (25, 100, 200, 300mL optional), 24 x 90mL
amino acid containers (250mL optional), interchangeable 15, 45, 105mL
measuring/preactivation vessels, 2 x 5L solvent bottles, 1 x 2L solvent
bottle, 2 x 0.5L reagent bottles, 1 x 2L deprotection solution bottle
• XC4 – 4 x 50mL RVs (25, 100, 200, 300mL optional) ), 24 x 90mL
amino acid containers (250mL optional), interchangeable 15, 45, 105mL
measuring/preactivation vessels, 2 x 5L solvent bottles, 1 x 2L solvent
bottle, 2 x 0.5L reagent bottles, 1 x 2L deprotection solution bottle
• XC6 – 6 x 50mL RVs (25, 100, 200, 300mL optional) ), 24 x 90mL
amino acid containers (250mL optional), interchangeable 15, 45, 105mL
measuring/preactivation vessels, 2 x 5L solvent bottles, 1 x 2L solvent
bottle, 2 x 0.5L reagent bottles, 1 x 2L deprotection solution bottle
• XC III – 1 x 500mL RV (1, 2, 3, 5L optional), 4 x 0.5L amino acid
containers (larger sizes optional), solvent/reagent bottles vary to
customer specifications
• On-instrument cleavage capabilities
• 1 x 20L waste carboy with built in overflow sensors
• 1 cap with insert for waste carboy
• Computer/control system with printer
• Heating module (optional)
• Sonication module (optional)
• UV monitoring technology (optional)
SCALE
• XC I – 0.05 to 5 mmol
• XC II – 0.01 to 5 mmol (total)
• XC2 – 0.05 to 15 mmol (total)
• XC4 – 0.05 to 30 mmol (total)
• XC6 – 0.05 to 45 mmol (total)
• XC III – 5 to 150 mmol
WARRANTY
One-year limited parts and labor
6309 Shepherdsville Road
Louisville, KY 40228-1027, U.S.A.
Phone: 502.968.2223
Toll-free: 888.692.9111
Fax: 502.968.3338
Email: info@aapptec.com
www.aapptec.com
APPT1091 FocusXC Brochure_REV.indd 8 5/23/11 4:47 PM