Delta Vision Elite Microscope

More documents

Recommendations

Info

DeltaVision ImagingIlluminationDrosophila ovary - Image courtesy In SituHybridization Course, Cold Spring Harbor LaboratoryInsightSSI Solid State IlluminationThe InsightSSI illumination module incorporates novel light source technologies foroptimal performance.• Extremely stable and long lasting illumination• Electronic control provides instant on/off operation• Microsecond switching between wavelengthsUltimateFocus UltimateFocus automatically maintains the sample z-position regardless of mechanicalor thermal changes that can impact your experiment.• Exclusive, patent-pending design• Real-time compensation of stage drift• Focus control within 25 nm8 micron thick fixed cryosection of mouse small intestine- Image courtesy Paul Appleton, Wellcome TrustBiocentre, DundeeNow with Focus Assist! The Focus Assist feedback loop of UltimateFocus determinesthe distance between the objective and the coverslip. It guides the user to bring theobjective into the area of focus without using the eyepieces or a camera.Illumination UniformityEvery DeltaVision system utilizes our proprietary photosensor correction system.• Continuously measures the excitation light output to ensure data integrity• Provides automatic image correction for intensity fluctuations as required forquantitative imagingImaging of 100 micron slices of autumn maple leaf- Image courtesy Kyla Teplitz and Katie Buchanan,Applied PrecisionDifferential Interference Contrast (DIC)DIC light microscopy produces finely detailed high contrast images to rapidly visualizethe state of the cell. DIC combined with epifluorescence provides more informationwithin context of the sample.• Quickly identifies healthy cells within a large population• Establishes focal plane to minimize photodamage to cells• Monitors cell viability during time-lapse experimentsMitochondria trafficking in arabidopsis root hairs- Image courtesy Naohiro Kato, 3D Microscopy ofLiving Cells
Laser ModuleX4 Laser ModuleThe X4 Laser Module combines up to four lasers thatare active simultaneously for increased performance.The increased laser line selection allows greaterflexibility in the use of fluorescent dyes and proteins.• More powerful lasers for shorter exposure timesincrease the accuracy of photokinetic measurements• Specialized optics generate a near diffractionlimitedspot size to target a measurable region ofinterest• Rapid laser switching enables combinations ofmultiple lasers to photoactivate and photobleach thesample within the same experimentMulti-Line TIRFTotal Internal Reflection Fluorescence (TIRF) microscopyis a specialized technique used to image sampleswithin 100-200 nm of the coverslip surface. Becauselight travels different distances, the challenge of TIRFis to ensure that all laser lines penetrate the sampleto the same depth. Applied Precision has solved thislimitation by automating several key components tobring the lasers to the same focal plane.• Performs chromatic correction of each wavelength tothe same penetration depth at the coverslip• Increases imaging options using four lasers in ournew X4 Laser Module• Flexible experiment design enables the combinationof photokinetic applications (PA-GFP) with TIRFimagingPhotoactivation/Photokinetics/FRAP/FLIPPhotoactivable and photoconvertible proteins give theuser the ability to take a more active role in expandingthe type of data collected. The sub-diffraction limitedspot size of the X4 Laser Module enables exquisitecontrol over the target area for quantitative results.• Photoactivation to initiate fluorescence of a dye orprotein to act as a marker within a larger population• Photokinetics to precisely calculate the rate anddirection of movement of the fluorophore of interest• Fluorescence Recovery after Photobleaching (FRAP)to photobleach a region of interest and measure therecovery of fluorescence in the bleached area• Förster/Fluorescence Resonance Energy Transfer(FRET) to characterize the interactions of proteins thatare known to associate with each other15Epifluorescent (green) and TIRF (red) images of zyxin:GFP localized to focal adhesions inHeLa cells
Page 1 and 2: The DV Elite Imaging System
Page 3: The TruLight Illumination SystemTh
Page 7 and 8: Deltavision InnovationDeconvolution

Delta Vision Elite Microscope

Create successful ePaper yourself

Delete template?

Save as template?