(GORO) Abubakar Ahmed - Ijsidonline.info

Abubakar Ahmed Hamidu et al., IJSID, 2012, 2 (1), 61-66concentrated hydrochloride acid, acetone and petroleum ether. Other materials used included laboratory apparatus,chromatographic tank and distilled water.MethodsThe samples were dried under room temperature and grounded to powder for further analysis. The method of coldextraction was adopted for this investigation.(A) Solution Preparations(i) Preparation of IRON II CHLORIDE solution (FeCl 3)In this preparation, 5 grams of FeCl 3 was weighed and dissolved in 100cm 3 volumetric flask. This was then made tomark and allowed to cool for 30 minutes.(ii) Preparation of 10% Potassium SolutionIn this preparation, 10 grammes of potassium hydroxide (KOH) was weighed and dissolved in 100cm 3 volumetricflask. The solution was vigorously shaken until the pellets dissolved completely. This was made to mark.(iii) Preparation of 10g Sodium Hydrocide SolutionTo achieve this, 10 grammes of NaOH was weighed and dissolved in 100cm 3 volumetric flask. This was vigorouslyshaken until all the pellets dissolved completely and was made to mark using distilled water.(B) Extraction ProcedureUsing the method of cold extraction as earlier mentioned, the two different plant materials i.e. the back and leaveswere grounded to powder form, 50 grammes of each of the plant materials i.e. back and leaves was weighed separately. Thesewere then separately soaked in a round bottom flask using acetone as the solvent. The conical flask was sealed and kept for aperiod of 48 hours i.e. approximately two days. The soaking was to allow the flavonoids to be absorbed by the solvent. After aperiod of 48 hours, the soaked sample was shaken and filtered into a 250ml beaker using Whatman filter paper 1. The filtratewas dried in an Air Oven. Further extraction was also done on the plant materials i.e. back and leaves using methanol andwater as solvents. The same procedure as above was followed in each case. The procedure of extraction was repeated for atleast three times in each case.(C) Photochemical Screening of Plant ExtractPlant material extracts from the back and leaves were obtained with acetone, methanol and water respectively. Thesewere all evaluated for the presence of the following below: -i. Flavonoidii. Phenolicsiii. Flavoneiv. Phenolv. Saponinsvi. Glycosides(D) Preparation of ExtractThe preparation of the plant material extract was done by weighing 0.5grammes of the solid extract. This weight wasthen dissolved in 5ml distilled which was used for the phytochemical screening tests.International Journal of Science Innovations and Discoveries, Volume 2, Issue 1, January-February 201263