Bioinformatics History and Introduction - Chagall

Sequence matching: problems• miRNAs are very small (21-22nt)– Enormous number of potential targets withcomplementary sequence– BLAST does not scale.• Low-complexity sequences– Signal to noise problem• Standard sequence analysis packages generally notapplicable– Looking for complementarity, not similarity• i.e. A:U G:C not A:A G:G etc.– Wobble pairing permitted• G:U and U:G base pairs• Small number of known cases to work with24 January 2013 Luce Skrabanek