Bioinformatics History and Introduction - Chagall

Controls• Shuffled sequences - have fewer matches than the realmiRNA• Selected so as to preserve all relevant compositional features– Expected frequency of seed matches to the UTR dataset– Expected frequency of matching to the 3’ end of the miRNA– Observed count of seed matches in the UTR dataset– Predicted free energy of the RNA duplex• Each shuffled control sequence also has the same length andbase composition as the parent• Signal:noise ratio = 3.2:1– 5.7 targets with real miRNAs versus 1.8 targets foundwith control sequences = ~3.9 “real” targets per miRNA– Approximately a 31% FP rate24 January 2013 Luce Skrabanek