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Seema Singh et al., IJSID 2011, 1 (2), 247-254Table 1. Effect of different combinations of hormones on multiple shoot regeneration from cotyledonary and hypocotylexplants in Brassica comprastis (l.) var. sawarnaMedia (MS media+phytohormone)Cotyledonary node% ofresponsiveexplants Shoots/explant Shoot length% ofresponsiveexplantsHypocotylShoots/explantShootlengthBAP (0.5 mg/l)- a 55 1.90 ± 0.57 3.57 ± 0.41 36 1.30 ± 0.48 2.12 ± 0.68BAP (1.0 mg/l)- b 64 2.00 ± 0.82 3.29 ± 0.71 28 1.40 ± 0.52 2.25 ± 0.79BAP (1.5 mg/l)- c 48 2.60 ± 0.70 2.34 ± 0.61 40 1.90 ± 0.74 2.42 ± 0.75Kn (0.5 mg/l)- d44 1.40 ± 0.52 2.65 ± 0.59 38 0.90 ± 0.57 1.08 ± 0.33Kn (1.0 mg/l)- e 56 2.10 ± 0.57 1.73 ± 0.37 41 1.70 ± 0.48 1.00 ± 0.36Kn (1.5 mg/l)- f 51 1.30 ± 0.48 1.50 ± 0.31 58 1.20 ± 0.42 1.14 ± 0.30BAP+Kn (0.5 mg/l + 1.0 mg/l)- g72 3.10 ± 0.88 3.41 ± 0.63 55 1.80 ± 0.79 2.40 ± 0.64BAP+Kn (1.0 mg/l + 1.0 mg/l)- h 74 4.40 ± 0.84 2.31 ± 0.66 57 2.00 ± 0.67 2.81 ± 0.76BAP+Kn (1.0 mg/l + 0.5 mg/l)- i 58 3.00 ± 0.82 2.95 ± 0.76 61 1.80 ± 0.79 2.10 ± 0.67BAP+Kn+NAA (1.0 mg/l+1.0mg/l + 1.0 mg/l)- j 68 2.70 ± 0.48 3.58 ± 0.87 51 1.40 ± 0.52 3.26 ± 0.56BAP+Kn+IAA (1.0 mg/l + 1.0mg/l + 1.0 mg/l)- k 85 15.40 ± 0.70 4.46 ± 0.85 61 5.50 ± 1.08 2.35 ± 0.85BAP+Kn+IAA (2.0 mg/l + 1.0mg/l + 1.0 mg/l)- l 80 5.80 ± 0.79 3.08 ± 0.52 56 3.90 ± 0.88 2.06 ± 0.54BAP+Kn+IAA (3.0 mg/l + 1.0mg/l + 1.0 mg/l)-m 74 5.40 ± 0.70 2.22 ± 0.43 48 5.40 ± 1.07 2.01 ± 0.68BAP+Kn+IAA (1.0 mg/l + 1.0mg/l + 2.0 mg/l)- n 63 3.90 ± 0.88 5.58 ± 0.89 49 3.10 ± 0.74 3.39 ± 0.77BAP+Kn+IAA (1.0 mg/l + 1.0mg/l + 3.0 mg/l)- o 73 3.30 ± 0.82 5.22 ± 0.84 43 1.60 ± 0.70 4.65 ± 0.73BAP+Kn+IAA (1.0 mg/l + 2.0mg/l + 1.0 mg/l)- p 69 5.30 ± 0.67 2.94 ± 0.59 50 3.00 ± 0.82 2.51 ± 0.60BAP+Kn+IAA (1.0 mg/l + 3.0mg/l + 1.0 mg/l)- q 81 2.70 ± 0.48 2.83 ± 0.55 49 2.70 ± 1.06 2.27 ± 0.58ANOVA F (DF=16,153) - 215.45 ** 29.44 ** - 34.11 ** 19.17 ****- p0.05**- p
Seema Singh et al., IJSID 2011, 1 (2), 247-254Fig. 1. Surface plot showing relative association of shoot length with % of responsive explants and shoots/explantin cotyledonary node.Fig. 2. Surface plot showing relative association of shoot length with % of responsive explants and shoots/explantin hypocotyl.International Journal of Science Innovations and Discoveries VOL1, Issue 2, September-October 2011251
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