improved protocol for shoot regeneration in ... - Ijsidonline.info

Seema Singh et al., IJSID 2011, 1 (2), 247-254and full strength media, supplemented with IBA (0.1mg/L) and NAA (1.0 mg/L), produced roots andconsequently the plantlets could be developed.CONCLUSIONIt is apparent from these results that MS media supplemented with BAP(1mg/L)+ Kn(1mg/L)+IAA(1mg/L) is more efficient for multiple shoot regeneration and it may be utilized in in-vitroimprovement program of Brassica campestris(L.) var. sawarna because in Brassica species organogenesisresponse are species and genotype specific 13 .REFERENCES1. Ali H, Ali Z, Ali HA, Mehmood S, Ali W (2007). In-vitro regeneration of Brassica napus L., cultivars (Star, Cyclone andWestar) from hypocotyls and cotyledonary leaves. Pakistan Journal of Botany 39(4), 1251-1256.2. Glemelius K., 1984. High growth rate and regeneration capacity of hypocotyl protoplast in some Brassicaceae, PhysiologyPlant 61:38-44.3. Lazzeri, P.A., Dunwell J.M., 1984 a. In-vitro shoot regeneration from seedling roots segments of Brassica oleracea var.Italica .Annuls. Botany 54, 351-361.4. Lazzeri, P.A., Dunwell J.M., 1984 b. Establishment of isolated root culture of Brassica species and regeneration fromcultivated roots segments of Brassica oleracea var Italica Annuls Botany 54, 351-361.5. George, L., Rao, P.S., 1980. In-vitro regeneration of mustard plant (Brassica juncea var.RAI-5) on cotyledon explants fromnon-irradiated, irradiated and mutagen treated seeds. Annuls Botany46,107-112.6. Chi GL, Pua E C, 1991. Role of ethylene on de-nova shoot regeneration from cotyledonary explants of Brassica campestrisspp. pekensis (Lour) oisson in –vitro. Plant Physiology 96,178-183.7. Dunwell, J.M., 1981. In-vitro regeneration from excised leaf disc of Brassica species. Journal of Experimental Botany32,789-799.8. Burbulis N, Blinstrubiene A, Kupriene R, Jonytiene V, Rugienius R, Staniene G, (2009). In- vitro regeneration of Brassicanapus L. shoots from hypocotyls and stem segments. Zemdirbyste- Agriculture 3, 176-1859. Murashige, T., Skoog. F., 1962. A revised medium for rapid growth and bioassay with Tobacco tissue cultures. PlantPhysiology 15, 473-497.10. Stewart, C.N., Adang, M.J., Ali, J.A., Wetal. 1996. Insect control and dosage effect in transgenic canola containing a syntheticBacillus thuringenensis cry IAC gene. Plant Physiology 112, 115-120.11. Kamal, G.B., Asadollah, A., 2007. Effect of genotype explant type and nutrient medium components on canola (Brassicanapus L.) shoot in –vitro organogenesis. African Journal of Biotechnology.67, 861-897.12. Koh, W.L., Loh, C.S., 2000. Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid cycling Brassicanapus. Plant Cell Report 19, 1177-1183.13. Dietert, M.F., Barron, S.A., Ocvodar, 1982. Effect of genotype on in-vitro culture in genus Brassica. Plant Science Letters26,233-240.International Journal of Science Innovations and Discoveries VOL1, Issue 2, September-October 2011254