September 2009 - Wadsworth Center

334a (Genebank accession number: AJ249484) for ITS1 and C. dubliniensis YN57-151205 (Genebankaccession number: DQ355938) for ITS2 region.Comments: This specimen was validated in the current test event. This is the second time a C.dubliniensis was validated in NYSDOH Mycology PT program. This specimen was sent out earlier as aneducational specimen in the Mycology PTP October 1997 event, and as a test specimen in October 2000,June 2003, May 2005, January 2007, and September 2007 PT events. In the current test event, about 85%laboratories were able to identify C. dubliniensis, which is a notable improvement over last validatedevent. As summarized earlier in this section, a number of physiological differences could be used todistinguish these two closely related Candida species.Sequences alignment:Query 1 TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACATGTGTTT 60||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||AJ249484 1 TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACATGTGTTT 60Query 61 TGTTTTGGACAAACTTGCTTTGGCGGTGGGCCTCTACCTGCCGCCAGAGGACATAAACTT 120||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||AJ249484 61 TGTTTTGGACAAACTTGCTTTGGCGGTGGGCCTCTACCTGCCGCCAGAGGACATAAACTT 120Query 121 ACAACCAAATTTTTTATAAACTTGTCACGAGATTATTTTTAATAGTCAAAACTTTCAACA 180||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||AJ249484 121 ACAACCAAATTTTTTATAAACTTGTCACGAGATTATTTTTAATAGTCAAAACTTTCAACA 180Query 181 ACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC 218||||||||||||||||||||||||||||||||||||||AJ249484 181 ACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC 218Alignment of primary sequence of the ITS1 regions of C. dubliniensis M 334a and PT specimen C. dubliniensis NYSDOH0907.Query 1 CATCGATGAAGAACGCAGCGAAATGCGATACGTAATATGAATTGCAGATATTCGTGAATC 60||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ355938 158 CATCGATGAAGAACGCAGCGAAATGCGATACGTAATATGAATTGCAGATATTCGTGAATC 217Query 61 ATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGC 120||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ355938 218 ATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGC 277Query 121 GTCGTTTCTCCCTCAAACCCCTAGGGTTTGGTGTTGAGCAATACGACTTGGGTTTGCTTG 180||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ355938 278 GTCGTTTCTCCCTCAAACCCCTAGGGTTTGGTGTTGAGCAATACGACTTGGGTTTGCTTG 337Query 181 AAAGATGATAGTGGTAAGGCGGAGATGCTTGACAATGGCTTAGGTGTAACCAAAAACATT 240||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ355938 338 AAAGATGATAGTGGTAAGGCGGAGATGCTTGACAATGGCTTAGGTGTAACCAAAAACATT 397Query 241 GCTAAGGCGGTCTCTGGCGTCGCCCATTTTATTCTTCAAACTTTGACCTCAAATCAGGTA 300||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ355938 398 GCTAAGGCGGTCTCTGGCGTCGCCCATTTTATTCTTCAAACTTTGACCTCAAATCAGGTA 457Query 301 GGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGA 340||||||||||||||||||||||||||||||||||||||||DQ355938 458 GGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGA 49734