2. MATERIALS & METHODS2.1 Light-trappingLight trap was used to sample nocturnal insects. The trap consists of a vertical whitesheet (2 X 2 m) illuminated by a 250W mercury-lithium bulb. The trap was set up inan open area facing the forest reserve, from 7:00 to 9:00 p.m. A GPS (Model: GarminGPSMAP 60CSx) was used to determine the coordinates of each sampling site.Temperature and humidity were taken with a digital gadget from Oregon Scientific(model no. ETHG-912).To evaluate diversity of the sampling area, insect species and individuals (≥ 5 mm)within the 1 X 1 m square of the white cloth were enumerated from 8:30 to 9:00 pm.This is a rapid biodiversity assessment method because by the end of the samplingtime, species and individual numbers can be obtained, and the data can be used tocalculate diversity indices, i.e. Shannon Wiener, Simpson and Fisher Alpha, using theSpecies Diversity & Richness version IV (SDR 2006). This method is simple, fast andcan be carried out by non-insect specialist. To avoid compounding human error, thesame staff was assigned to count the species and individual numbers throughout thesampling period, and also for other sampling sites. Light-trapping sites are shown inTable 2.Table 2: Light-trapping sites at Kawag Base Camp, USM FRs.Sampling site Coordinates Elevation(m)A1 N 05 o 03.066’(Kawag riverside E 117 o 58.278’area)A2(Kawag BaseCamp entrance)A3(Ali Baba trail)N 05 o 03.006’E 117 o 58.846’N 05 o 02.930’E 117 o 58.880’2.2 Insect specimens and identificationTemp.( o C)Humidity(%)SamplingdateRemarks91 26 87 13 Feb Fine weatherwithout moon114 24 89 14 Feb Fine weatherwithout moon100 25 91 15 Feb Fine weatherwithout moonIn this survey, focus was given to nocturnal insects only. Photographs were taken witha DSLR Nikon D300 and a compact Nikon Coolpix to facilitate identification. Onlyselected insects were sampled but photographs of most insects sighted were taken forrecord purposes.Specimens and photographs were identified based on the FRC Entomology Collectionand various reference materials, e.g. Otsuka (1988 & 2001) and Corbet & Pendlebury(1992) for butterflies; Holloway (1983, 1985, 1986, 1988, 1989, 1993, 1996a, 1997,1998a & b, 1999, 2001, 2003, 2005, 2008, 2009 & 2011) and Robinson et al. (1994)for moths; Fujita (2010), Makihara (1999) and Tung (1983) for beetles.6
3. RESULTS & DISCUSSION3.1 Nocturnal insect diversityThe nocturnal insect diversity at the Kawag Base Camp during the sampling periodwas relatively low compared to other forest reserves as indicated in Table 3. Being afairly good logged over forest, it was expected to yield much better diversity results.Moreover, various species of interesting insects, especially macro moths were seenattracted to the lights within the Kawag resthouse, staff quarters and office at night.The low nocturnal insect diversity duringthe sampling period was partly due to theproliferation of a few hymenopteran species,especially flying ants (Figure 3). It was partof the nuptial flight and mating process ofthe ants. It is not uncommon for one colonyto release hundreds or thousands of youngwinged males and queens at any one time.Many are attracted to the light. The highabundance of the ant species is reflected inthe species-rank abundance curves inFigure 4, especially at Site A1 and A3.Figure 3: The mating process of aPolyrhachis ant species during lighttrapping.Table 3: Insect diversity within a one-square-metre, as sampled through lighttrappingat Kawag Base Camp, USM FRs (Sites A1, A2 & A3) compared to selectedsites of Rafflesia, Milian Labau, Bukit Hampuan and Gn. Lumaku F.Rs.No. Sampling site Species Ind. Shannon Simpson Fisher Alpha1 A1 75 139 3.56 12.31 66.442 A2 51 63 3.83 102.80 124.803 A3 55 97 3.16 7.68 52.654 Rafflesia(Site B)5 Milian Labau(Site 1)6 BukitHampuan(Site 2)7 Gn. Lumaku(southern part -Site 5)61 67 4.06 245.67 330.0679 122 4.05 45.56 97.03119 142 4.61 111.23 346.35124 163 4.56 90.43 236.607