Detection and Analysis of the New “Designer Steroids”
Detection and Analysis of the New “Designer Steroids”
Detection and Analysis of the New “Designer Steroids”
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Androsterone/ Etiocholanolone (A/E)Fast GC - ~ 1 second separationStanozolol Metabolites ConfirmationA & E are main urinary <strong>and</strong>rogen metabolites2.49 2.51Androsterone - 5α metaboliteEtiocholanolone - 5β metaboliteAAEEEAHighNormalLowPossible use <strong>of</strong> 5α-DHT(5α-dihydrotestosterone)Possible use <strong>of</strong> 5αreductaseinhibitors,masking agentsMinutesDifferentiation <strong>of</strong> Natural <strong>and</strong> Syn<strong>the</strong>ticEndogenous Steroids by IRMS –Isotope Ratio Mass Spectrometry• The ratio <strong>of</strong> two stable carbon isotopes 13 C<strong>and</strong> 12 C allow to discriminate endogenousfrom chemically identical, but syn<strong>the</strong>ticsteroids in urine.• Syn<strong>the</strong>tic substances usually exhibit differentcomposition <strong>of</strong> 13 C <strong>and</strong> 12 C isotopes thanmaterial <strong>of</strong> exclusive biological originIRMS for carbon isotope measurementGC separationOn-line combustion to CO 2<strong>and</strong> HO 2MS analysis <strong>of</strong> CO 2• Ion abundances are measured at• M/Z = 44 represents 12 C• M/Z = 45 represents 13 C•M/Z = 46 correction for <strong>the</strong> Oxygen isotopes 17 O <strong>and</strong> 18 O
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