New Feed Enzyme Development - The Poultry Federation

More documents

Recommendations

Info

September 2009, ChemGen Corp.Figure 4 –Pure mannanase performance in necrotic enteritismodelCage trial for 21 days conducted at SPRwith the necrotic enteritis model. All treatments infected with E. maxima,E. acervulina, and C. perfringens. Medicated treatment contained Salinomycin 60g/ton and BMD 50 g/ton.Different lower case letters on bars signify statistical significant difference (P < 0.05). Hemicell® or β‐mannanasewere added at 100 MU/ton +/‐ 5 MU. Purified mannanase is as described in Figure 3.Hemicell® Feed Enzyme and Innate Immune ResponseLegumeslike soybeans contain galactomannan in a portion of the seed called the endospermillustrated in Figure 5. The domesticated US soybeans have a very small remaining endosperm.In corn‐soybean diets the galactomannan content is derived essentially all fromthe soybeanmeal (33). The 44% protein soybean meals have low galactomannan (estimatedaverage 1.61%) and even lower in48% protein meals made with dehulled beans (estimated average 1.26 %)since much of the endosperm sticks to the tothe hull . The active ingredient mannanase onlylowers the molecular weight of galactomannan producing primarily mannose oligosaccharidesas illustrated Figures6 and 7. The extent of α‐1,6‐ side chains on the β‐1,4‐mannan backboneaffects the mannan’ssolubility and also the degree of digestion. A higher modification levelincreasess solubility, but also causes somewhat higher degree of polymerization(dp) for a limitdigest. On the otherhand β‐1,4‐mannans with a low galactose modification have low solubilityin water,and water solubility is necessary for efficient β‐mannanase action. Thegalactomannan digestion in Figure 7 uses highy soluble Locust bean gum galactomannan thathas a similar high degree of α‐1,6‐ galactose side chain content as soy galactomannan. It can beseen thatt MOS with dp ranging from 2 to well over 10 isproduced. No fragments are seenwithout mannanase addition.D.M. Anderson and H.Hsiao, New Feed EnzymesPage 10
September 2009, ChemGen Corp.Figure 5 – Endosperm and galactomannan in LegumesenPhotographs provided by Dr. James A. Lackey. Seed types are (a) Kudzu; (b) wild soybean; (c) US domesticatedsoybean; (d) Guar. The endosperm composed largely of galactomannan is labeled “en”. The cotyledon or embryois rich in protein and is increased in size in domesticated soybean.Figure 6‐Hydrolysis of Legume galactomannan by endo‐1,4‐β‐D‐mannanase (EC 3.2.1.78) of Hemicell®Legume galactomannanα-1,6-galactoseβ-1,4-mannoseHydrolysis SitesnFigure 7 – Oligosaccarides produced from Locust Bean gum galactomannan1 2 3 4 5 6dp 10dp 4dp 2Acrylamide electrophoresis of oligosaccharides deriviatized withfluorescent ANTS to create negatively charged molecules thatare separated by size (40). Gel composed of 28% acrylamideand 2% bisacrylamide was used and was photographed underUV light. Lane 1 – maltodextrin with degree of polymerization(dp) of 4‐10; Lane 2‐ maltodextrin dp 2‐4; lane 3 – glucose ;Lane 4 – Locust bean gum; Lane 5 and 6– Locust bean gumgalactomannan digested by Hemicell® mannanase. Glucose runsin the dye front including non reacted ANTS.D.M. Anderson and H. Hsiao, New Feed Enzymes Page 11
Page 3: September 2009, ChemGen Corp.protei
Page 9: September 2009, ChemGen Corp.In a 4
Page 17 and 18: September 2009, ChemGen Corp.First
Page 19 and 20: September 2009, ChemGen Corp.Figure
Page 21 and 22: September 2009, ChemGen Corp.Table
Page 23 and 24: September 2009, ChemGen Corp.Table
Page 25 and 26: September 2009, ChemGen Corp.Figure
Page 27 and 28: September 2009, ChemGen Corp.REFERE
Page 29 and 30: September 2009, ChemGen Corp.37. Ja

New Feed Enzyme Development - The Poultry Federation

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?