New Feed Enzyme Development - The Poultry Federation

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September 2009, ChemGen Corp.Table 7‐ Chicken 21 day broiler trial at SPR with β‐1,3‐glucanase to test AGP responseTreatment WAFCStat.P=0.05AGPmg/LStat.P=0.05control 1.474 a 255.4 aHemicell 1.390 ab 184.1 b1,3- glucanase 1.264 c 157.7 bOne‐day‐old male Cobb X Cobb chicks were obtained from Cobb‐Vantress hatchery, Cleveland, GA on April 20,2006. Chicks were raised in Petersime battery cages. Space per animal was 0.631 sq.ft/bird (588 sq.cm/bird), 8birds per cage. The feeder space per bird was 8 birds/43 cm x 6.8 cm feeder. Thermostatically controlled gasfurnace/air conditioner maintained uniform temperature. Even illumination was provided. Cages were blocked bylocation in the battery with block size equal to treatments (21 cages per block and 3 replications per treatment).Feed and water were given ad libitum. On Day 21, approximately 3 mls of blood were collected from each bird.Blood was placed into a EDTA coated tube and lightly mixed. Tubes were slowly centrifuged and then serum wasremoved. Serum samples were placed into tubes with caps labeled with cage number. Serum was frozen and sentto ChemGen for AGP analysis by radial immunodiffusion.Table 8 ‐ Turkey Hen trial, 42 daysTreatment WAFC Stat AGP Statcontrol 1.520 a 214.8 aHemicell 1.502 1 ab 177.2 abβ-1,3-glucanase 1.465 c 172.1 bβ- 1,3-glucanase + Hemicell® 1.402 1 d 171.3 bOne‐day‐old female turkey poults were obtained from Tar Heel Hatchery, Raeford, NC. The birds were kept in 80pens each having an area of 5 x 10 = 50 ft 2 , with built up poultry litter topped with fresh pine shaving as bedding for athickness of approximately 4 inches. There were 10 pens per treatment and 40 hens/pen. The stocking density, aftersubtracting out for equipment, was 1.16 ft 2 /turkey‐hen. All basal feeds were manufactured at Purina Feed Mills,Gainesville, GA. Coban, at 90 g/t, was added to the pre‐starter feeds by SPR. Turkey AGP was determined byimmunodiffusion as described in the materials an methods, but the turkey AGP kits have been discontinued. 1 Note– data for Hemicell® teatment and β‐ 1,3‐glucanase + Hemicell® treatment are the average result from duplicatedtreatments.An additional research trial at Southern Poultry Research was recently completed. The basic dietcompositions, crude protein, metabolic energy, and cost for each feeding period are shown in Table 8.Data from 84 days is plotted in Figures 18‐20. The corn control diet is an optimal corn soy control withhigh caloric content. The objective was to see if the novel β‐1,3‐glucanase or Hemicell® could improvediets containing DDGS. Thus there are two more diets, one with DDGS plus energy equivalent to thecorn/soy diet and a DDGS diet with low caloric content that that was expected to be replaced by enzymeuse. The enzymes were added to the DDGS low energy diet. In general the enzymes are improving thenegative DDGS control up to the level of the DDGS positive control, except at 42 days where thedifference between the DDGS positive control and corn soy control for some reason became quite large.From these results it appears the addition of β‐1,3‐glucanase did not provide significantly better feedconversion than the than the β‐mannanase alone in the diets.D.M. Anderson and H. Hsiao, New Feed Enzymes Page 22
September 2009, ChemGen Corp.Table 8 –Basic diet composition for SPR100708 Turkey hen trialCornControlPos.ControlNeg.ControlPrestarter 0‐3 weeksCorn 41.9 39.7 40.3SBM 45.9 43 42.2Fat 1.2 1.2 0.5Poultry Meal 6 6 6DDGS ‐ 5 5.7CP 29 29 29ME 2850 2850 2817Cost ($)/ton 335.18 330.07 325.19Starter 3‐6 weeksCorn 43 38.7 40.4SBM 43.6 37.8 36.1Fat 2.7 2.7 0.5Poultry Meal 6 6 6DDGS ‐ 10 12.3CP 28 28 28ME 2950 2950 2850Cost ($)/ton 338.97 329.14 314.1Grower 6‐9 weeksCorn 49.7 43.3 46SBM 38.3 29.6 26.8Fat 4.2 4.3 0.5Poultry Meal 4 4 4DDGS ‐ 15 18.8CP 24.5 24.5 24.5ME 3100 3100 2928Cost ($)/ton 328.44 313.24 287.94Finisher 9‐12 weeksCorn 60.1 53.6 56.8SBM 28.2 19.6 16.2Fat 4.8 4.9 0.5Poultry Meal 4 4 4DDGS ‐ 15 19.4CP 20.5 20.5 20.5ME 3250 3250 3050Cost ($)/ton 311.61 29.24 266.64D.M. Anderson and H. Hsiao, New Feed Enzymes Page 23
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