New Feed Enzyme Development - The Poultry Federation

September 2009, ChemGen Corp.proteins (APP) in blood provides a relatively quick screen to identify other potentially useful enzymesubstratecombinations. APP levels are generally easy to measure since they amplify (or decrease)rapidly upon innate immune system stimulation (14).In this review, example data is presented showing the impact of β‐mannanase on serum AGP levels, andthe inverse relationship between serum AGP and growth performance. The rational for a new feedenzyme we have developed is described and how it was initially validated as a good candidate by serumacute phase protein screening. The new enzyme’s properties are highly analogous to the qualities of β‐mannanase as a feed enzyme. Both degrade PAMPs in feed that are recognized by hosts PRRs, and bothenzymes we believe increase feeding efficiency primarily through the elimination of a counterproductiveinnate immune response. This novel enzyme for feed has the potential to become another widelyaccepted feed enzyme supplied by ChemGen.ABBREVIATIONSTERMMEANINGAAFCOAmerican Association of Feed Control OfficialsAGPα1‐acid glycoproteinamylase α‐amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41);glucoamylase (EC 3.2.1.3); β‐amylase (EC 3.2.1.2)ANTS8‐anino‐1,3,6,‐naphthalene trisulfonic acidAPPacute phase proteinBDGBrewer’s dry grainsBMDbacitracin methylene disalicylatecfucolony forming unitsChemGen MU million ChemGen units = 4000 IUChemGen Unit (U) 250 International enzyme units (IU)CMPCarboxymethyl pachymanCPCrude proteinCRDcarbohydrate binding domainsDC‐SIGNdendritic cell intercellular adhesion molecule‐3‐grabbing non‐integrinDDGSDistillers dried grain and solublesdpdegree of polymerization (as in the # of sugars in a sugar polymer)FC (or FCR)feed conversion (feed injested/weight gain)GPI‐linkedglycosylphosphatidylinositol linkedHemicellulase enzyme that hydrolyzes a hemicellulose, but historically, a nameparticularly applied to β‐mannanaseHemicellulose any of several non‐celluose carbohydrate polymer components of plantcellsIGF‐Iinsulin like growth factor IIGF‐IIinsulin like growth factor IIIUInternational Enzyme Unit ( 1.0 micromole/minute enzyme reaction)MOSmannose oligosaccharidesMRmannose receptorNEFAnonesterified fatty acidsPAMPPathogen Associated Molecular PatternD.M. Anderson and H. Hsiao, New Feed Enzymes Page 3