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#75 Wnt5a Induced Tumorigenic Behaviors of Enamel Epithelium Cell via The Non-CanonicalWnt5a/JNK Pathway.W.SUKARAWAN, D. SIMMONS, C. SUGGS, and J.T. WRIGHTDental Research Center, University of North Carolina - Chapel Hill, USAObjective: Ameloblastoma is the most common epithelial odontogenic tumor. It is locally invasive, persistentgrowth with high ability to produce marked deformity of the face. We previously demonstrated that high Wnt5aexpression was detected in the abnormal epithelial component of human ameloblastoma using microarray andimmunohistochemistry approaches. Moreover, overexpression of Wnt5a in enamel epithelium cell (LS-8)associated with several altered growth properties which were characteristic of tumorigenic cells. Wnt5a is knownto initiate at least three non-canonical pathways in different cell types depending on cell context. Method: In thisstudy, pharmacological inhibitors were used to determine the downstream pathway that was triggered by Wnt5a inenamel epithelium cell. Results: We demonstrated that specific JNK inhibitor (SP600125) and ROCK inhibitor(Y27632) were able to reverse the transformed phenotypes which we found in Wnt5a overexpressed enamelepithelium cell. We also found that when JNK1 or JNK2 were overexpressed in LS-8 cells, they exhibited thesimilar phenotype to Wnt5a overexpressed cell. Conclusion: Our results showed the involvement of JNK pathwayand Wnt5a in enamel epithelium cell and suggested that Wnt5a/JNK pathway might responsible for tumorigenicbehavior in enamel epithelium cell. Supported by NIH/NIDCR#76 The Effects of Surface Roughness on Expression of Genes Involved in Wnt Signalingpathway during Osteogenic DifferentiationJ. GUO, D.B. MENDONCA, G. MENDONCA, and L.F. COOPERDepartment of ProsthodonticsThe Wnt signaling pathway is a complex network of proteins most well known for their roles in embryogenesisand cancer. In human, there are at least 19 family members of Wnt proteins which act as ligands mediating Wntsignaling pathway. The roles of Wnt signaling pathway in normal physiological processes in adults have also beensuggested, including bone remodeling. Objective: The aim of the study is to investigate the effects of surfaceroughness on Wnt signaling pathway during the osteoblastic differentiation of human mesenchymal stem cells(hMSCs). Methods: Human MSCs were seeded onto the acid-etched titanium rough surface and smooth surface,and induced to differentiate along osteogenic pathway for 1 to 14 days. Total RNA and then the cDNAs weresynthesized from the cells collected after 1, 3, 7, and 14 days of osteogenic induction. The pathway-specific qRT-PCR arrays were used to study the expression levels of the genes involved in Wnt signaling pathway. Results: Oneday after osteogenic differentiation, several Wnt signaling pathway related genes were regulated on both roughand smooth surfaces, including upregulated LRP5, FZD 1, 2, 5, 7, beta-catenin, beta-catenin interacting protein 1,and et al. Several genes were downregulated including Wnt 1, 2, 6, 7A, 7B, 10A, 11, and SFZD4. The differenceof gene expression between cells from the rough surface and smooth surface were noticed after osteogenicinduction. Conclusions: Genes involved in Wnt signaling pathway were regulated during osteogenic differentiationof hMSCs on both rough and smooth titanium surfaces. Rough surface demonstrated more regulated genes thanthe smooth surface.46
#77 Impact of the Visible Presence of Third Molars on Periodontal Health in Middle-AgedAmericansE.L. FISHER, K.L. MOSS, E.S. OH, J.D. BECK, S. OFFENBACHER, and R.P. WHITE Jr.Departments of Oral & Maxillofacial Surgery, Periodontology and Dental ResearchObjective: Document the presence of 3rd molars in a population of middle-aged Americans and evaluate theimpact of the presence of 3rd molars on periodontal health on non-3rd molars in these same subjects. Methods:This cross-sectional analysis included 6,793 subjects ages 52-72 years who underwent a periodontal exam in the1996-1998 Dental Atherosclerosis Risk in Communities (ARIC) Study. Calibrated examiners measured probingdepths (PD) at six sites per tooth on all visible teeth noting clinical 3rd molar absence. Indicator variables forperiodontal pathology were periodontal probing depths at least 4mm (PD=4mm) and clinical attachment levels atleast 3mm (CAL=3mm). Outcome variables were demographic and clinical characteristics (mean periodontalprobing depths, % periodontal probing sites with PD>4mm, and % periodontal probing sites with CAL=3mm)grouped by tooth-type. Explanatory variables were the presence or absence of visible 3rd molars. Differences inoutcomes between subjects with and without 3rd molars were compared by Chi-square and t-tests withsignificance
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Page 34 and 35: #39 The Effects of Epidermal Growth
Page 36 and 37: #43 The Impact of Removal of Third
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