HAI_Price_List_2005 (Page 1) - LC column, HPLC column, HILIC ...

PROTEO 300PROTEO 300Robust BioseparationsPhases C4 and C18Particle Sizes 4.5 and 10µmPore Size 300ÅPore Volume 0.9mL/gmSurface Area 100m 2 /gm%Carbon (w/w) C4 = 3%, C18= 8%Phase type Monofunctional & fully endcappedSilica Class Type BGuide to PROTEO 300 Part NumbersRx-xxxx-W045 PROTEO 300 C4 5µmRx-xxxx-W185 PROTEO 300 C18 5µmSee Page 24 for complete Part Number informationProteo300 C18Manufacturer A300 C18NEWApplicationsLow pH operating conditions and highpH column washing steps are typicalbioprocessing operating conditions thatcan severely limit the lifetime andperformance of widepore columnstypically used for protein and peptideanalysis. Higgins Analytical’s newPROTEO300 has high capacity forenhanced resolution and is robustenough to withstand prolonged use atextreme pH (1.5 - 10).Stability Study Under ExtremelyAlkaline ConditionsExperimental:150 x 4.6mm columns were studied. Allexperiments were conducted onduplicate columns. Solutes used forperformance measurement weredimethylphthalate and fluorene.Step One:Column stability and measurementprecision was established for eachcolumn with multiple injections over18hrs under neutral conditions (70%MeCN/water, 1mL/min). All columnswere very stable. The chromatograms ofa column from each manufacturer at theend of the 18 hour period are shown onthe left hand side.Step Two:Each column was flushed with 70%MeCN/0.5N NaOH solution at 1mL/minfor one hour (23ºC). The columns werethen purged and equilibrated under thestandard neutral conditions thenretested. The center chromatograms arethe results of the first test after neutralequilibrationManufacturer P300 C18Step Three:After the second step, the neutral testconditions were maintained for one hourand a repeat injection was made. Theresults are illustrated in thechromatograms on the right hand side ofthe figure.While Higgins Analytical does notrecommend flushing any silica-basedHPLC column under such aggressiveconditions, these data show the superiorakaline stability of Proteo 300 C18.13