HAI_Price_List_2005 (Page 1) - LC column, HPLC column, HILIC ...

HAISIL 300 APPLICATIONSVery High Peak CapacityDepending upon the type of informationa protein chemist is seeking, the HPLCanalysis of a complex digest can take 1to 2 hours. The following chromatogramTryptic Map of BSAon HAISIL 300 C18 5µm 250x4.6mmP/N HS-2546-W185illustrates a high resolution tryptic digestmap of BSA on a 250x2.1mm HAISIL300 C18 5µm column indicates peakcapacities in excess of 100. Smallproteins, on the other hand can yieldfewer fragments, and if only a "fingerprint"of a digest is required, high peakcapacity columns can be a waste oftime.Fast Analysis of a Complex DrugDelivery SystemMost pharmaceutical QC laboratoriesare faced with rapidly increasingdemands for high throughput capability.A good example of one laboratory'sPeptide Map in 5 minutesThe figure below illustrates a very fastpeptide map of cytochrome-C. Theinformation that can be gleaned from thisfive minute profile is more than adequatefor many analytical requirements. Therobust 50x4.6mm HAISIL 300 C18column provides hundreds of runswithout performance loss even underthe aggressive 6ml/min flow conditionsused in this example.The cytochrome-C is a good example ofpushing a column beyond its optimumoperating conditions (motr yhan sixtimes optimum linear velocity) and stillbeing able to get desired results. Thesame column can be run under moreconventional conditions with a flattergradient to optain even higher resolutionseparations. The rhGH tryptic map belowwas done on a 5cm long column thatreplaced the conventional 25cm columnthat was used initially.The Role of Column Length inGradient SeparationsChanges in column length have a directand linear effect on theoretical plates andanalysis time in isocratic separations.Column length plays a less important role,however, in gradient techniques. The threechromatograms below illustrate how wellpacked short HPLC columns yield verysimilar peptide separations under identicalgradient conditions.For this study, the 2.1mm I.D. HAISIL 300C18 5µm columns ranged from 250 to100mm in length. While solvent andanalysis time savings may be minimal,column cost savings can be significantwhen using as short a column as possible.Short columns also exhibit lower pressureat high flow rates.250x2.1mmHAISIL 300 C18 5µmHS-2521-W185150x2.1mmHAISIL 300 C18 5µmHS-1521-W185approach to addressing a large numberof samples for analysis on a daily basis issummarized below. This novel drugdelivery system contains a small proteinand other components that span anorder of magnitude in molecular weight.All the compounds of interest wereeasily resolved in a single analysis with afast linear gradient on a 5cm longHAISIL 300 C18 5µm column.Fast Tryptic Map of rhGH50x4.6mm HAISIL 300 C18100x2.1mmHAISIL 300 C18 5µmHS-1021-W1851. Gly-Tyr2. Val-Tyr-Val3. Met Enkephalin4. Leu Enkephalin5. Angiotensin II7