HAI_Price_List_2005 (Page 1) - LC column, HPLC column, HILIC ...

HAISIL 100 APPLICATIONSHormone AnalogsLH-RH (Luteinizing Hormone - ReleasingHormone) Analogs are used for theinhibition of gonadal steroid production.An important application area is in thetreatment of prostate cancer.ConditionsHAISIL 100 C18 5µm 150x4.6mmSolvent A: Phosphoric acid, ammoniumsulfate aqueous bufferSolvent B: Acetonitrile.Linear gradient at 1mL/min254nm detectionAnalogs:A1: pGlu-His-Trp-Ser-Tyr-(d-Ser(but))-Leu-Arg-Pro-NHEtA2: pGlu-His-Trp-Ser-Tyr-d-Ala-Leu-Arg-Pro-NHEtA3: pGlu-His-Trp-Ser-Tyr-d-Leu-Leu-Arg-Pro-NHEtA4: pGlu-His-Trp-Ser-Tyr-d-Ala-Trp-Leu-Pro-NHEtA5: pGlu-His-Trp-Ser-Tyr-d-Trp-Leu-Arg-Pro-NHEtAlkaloidsA Peruvian vine named “Una de Gato”(Uncaria tomentosa) and commonlyreferred to as Cat’s Claw, has become apopular natural product source forherbal remedies. Enthusiasts claim theoxindole alkaloid extracts to be effectivein the treatment of a wide range ofdigestive and circulatory diseases as wellas other afflictions ranging from cancerto arthritis. Reputable providers of suchphytochemical containing formulationscarefully assay the alkaloid content of theextracts and formulations. Thechromatogram above illustrates baselineresolution of Cat’s Claw bark extractscontaining isopteropodine, pteropodine,mitraphylline, isomitraphylline,ryncophylline and isorynchophylline.Kinetic Measurements in DiseaseResearchResearchers at the University ofCalifornia, Santa Cruz have recentlypublished a novel HPLC method thatyields baseline separation of per-deuteratedand protonated lipoxygenases.The striking ability of a 250x4.6mmHAISIL 100 C18 column to resolvethese two very similar compounds isenabling researchers to study largecompetitive kinetic isotope effects inHuman 15-lipoxygenase catalysis.Protio/per-deutero ratio data obtainedby this HPLC method agreed withinexperimental error of data obtained byES-MS.The elucidation of these mechanisms isnoteworthy because of the increasingrole human lipoxygenases are playing ininflammatory diseases and cancergrowth regulation.Alkaloids from Cat’s Clawanalyzed on a HAISIL 100 C18 columnP/N HS-2546-M185Enzymatic Reaction Mixtures ofHuman 15-lipoxygenaseHAISIL 100 C18 250x4.6mm74.9% MeOH, 25% water, 0.1%HoAC1mL/min, 234nmP/N HS-2546-M185Economical Alternative for PICOTAG AnalysisOur HAISIL 100 C18 columns havedemonstrated superior resolution and lifetime for PICOTAG amino acid analysiswhen compared to considerably moreexpensive columns from other manufacturers.Besides good linearity and columnlifetime, the 150x4.6mm column yieldsvery efficient amino acid analysis asshown in the chromatogram above (P/NHS-1546-M185).Carbamate Pesticide Analysis250 x 4.6mm HAISIL 100 C18HAISIL 100 - the Separations WorkHorseThe 100Å pore size, monomeric bondedphase, and very high performance makeHAISIL 100 an ideal choice for separationsranging from small molecules suchas carbamate pesticides and pharmaceuticaldrugs to synthetic oligonucleotidesand small peptides. High efficiency,symmetrical peaks, and long column life ischaracteristic not only of the C8 andC18 reverse phase columns, but thenormal phase silica columns as well.5