flashBAC Manual - Oxford Expression Technologies

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Figure 3. Schematic of the <strong>flashBAC</strong> system for makingrecombinant baculovirusesOXFORD EXPRESSION TECHNOLOGIES 18
OXFORD EXPRESSION TECHNOLOGIES 19
Page 1 and 2: User GuideJanuary 2008OXFORD EXPRES
Page 3 and 4: flashBAC User GuideContentsPage1 Li
Page 5 and 6: 1 Limited Use Licence1 In this Lice
Page 7 and 8: 2 Kit ContentsAll reagents and mate
Page 9 and 10: 6 Safety Requirements1 These resear
Page 11 and 12: Figure 1. A rod-shaped baculovirus
Page 13 and 14: development of the baculovirus expr
Page 15 and 16: the gene of interest, under the con
Page 17: ie1 etc. Examples include pBacPAK8/
Page 21 and 22: • Sterile baculovirus transfer ve
Page 23 and 24: 6 Just before the end of the incuba
Page 25 and 26: affected by using semi-pure transfe
Page 27 and 28: Procedure:1 Prepare a 100-200 ml cu
Page 29 and 30: cycles. Upon freezing, the viral ti
Page 31 and 32: 8.3 Plaque assay to titre recombina
Page 33 and 34: convenient to prepare 0.5 ml diluti
Page 35 and 36: from each of the 35 mm dishes, by t
Page 37 and 38: plates give a titre of:25 x 10 6 x
Page 39 and 40: OXFORD EXPRESSION TECHNOLOGIES 39
Page 41 and 42: 9 Analysis of gene expression: a gu
Page 43 and 44: for 1 hour, remove the inoculum and
Page 45 and 46: Multiplicity of infectionIn order t
Page 47 and 48: 10 A Guide to Insect Cell CultureIt
Page 49 and 50: orbital shaker platform, whilst cel
Page 51 and 52: counting chamber (Figure 5), using
Page 53 and 54: Passaging suspension cultures of ce
Page 55 and 56: Table 3. Seeding densities of insec
Page 57 and 58: 11 Troubleshooting & FAQQ) Why are
Page 59 and 60: culture dishes within 1 h after pla
Page 61 and 62: Is the coding region of the gene do
Page 63 and 64: 11. Kitts P. A., Ayres M. D., Posse

flashBAC Manual - Oxford Expression Technologies

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