flashBAC Manual - Oxford Expression Technologies

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4 Ordering InformationTo order by post:Oxford Expression TechnologiesOxford Brookes UniversityHeadington CampusOxfordOX3 OBPUKTo order by email: sales@oetltd.comTo order by Fax: +44 (0) 1865 483250Fax form available at: www.oetltd.com5 Technical Assistance and Further InformationFor additional help or guidance please refer to theTroubleshooting section of this User Guide and/or thefrequently asked questions (FAQ) section of our website athttp://www.oetltd.com. If these resources areunable to help you, please contact us atinfo@oetltd.com. All technical assistance isprovided without charge and is given in good faith; we cannottake any responsibility whatsoever for any results you obtain byrelying on our assistance. We make no warranties of any kindwith respect to technical assistance or information we provide.OXFORD EXPRESSION TECHNOLOGIES 8
6 Safety Requirements1 These research products have not been approved forhuman or animal diagnostic or therapeutic use.2 Procedures described within this User Guide should onlybe carried out by qualified persons trained in appropriatelaboratory safety procedures.3 Always use good laboratory practice when handling thisproduct.WARNING: SAFETY PRECAUTIONS MAY NECESSARYWHEN HANDLING SOME OF THE PRODUCTS DESCRIBEDIN THIS USER GUIDE. PLEASE REFER TO THE MATERIALSAFETY DATA SHEET SUPPLIED BY THE APPROPRIATEMANUFACTURER.OXFORD EXPRESSION TECHNOLOGIES 9
Page 1 and 2: User GuideJanuary 2008OXFORD EXPRES
Page 3 and 4: flashBAC User GuideContentsPage1 Li
Page 5 and 6: 1 Limited Use Licence1 In this Lice
Page 7: 2 Kit ContentsAll reagents and mate
Page 11 and 12: Figure 1. A rod-shaped baculovirus
Page 13 and 14: development of the baculovirus expr
Page 15 and 16: the gene of interest, under the con
Page 17 and 18: ie1 etc. Examples include pBacPAK8/
Page 19 and 20: OXFORD EXPRESSION TECHNOLOGIES 19
Page 21 and 22: • Sterile baculovirus transfer ve
Page 23 and 24: 6 Just before the end of the incuba
Page 25 and 26: affected by using semi-pure transfe
Page 27 and 28: Procedure:1 Prepare a 100-200 ml cu
Page 29 and 30: cycles. Upon freezing, the viral ti
Page 31 and 32: 8.3 Plaque assay to titre recombina
Page 33 and 34: convenient to prepare 0.5 ml diluti
Page 35 and 36: from each of the 35 mm dishes, by t
Page 37 and 38: plates give a titre of:25 x 10 6 x
Page 39 and 40: OXFORD EXPRESSION TECHNOLOGIES 39
Page 41 and 42: 9 Analysis of gene expression: a gu
Page 43 and 44: for 1 hour, remove the inoculum and
Page 45 and 46: Multiplicity of infectionIn order t
Page 47 and 48: 10 A Guide to Insect Cell CultureIt
Page 49 and 50: orbital shaker platform, whilst cel
Page 51 and 52: counting chamber (Figure 5), using
Page 53 and 54: Passaging suspension cultures of ce
Page 55 and 56: Table 3. Seeding densities of insec
Page 57 and 58: 11 Troubleshooting & FAQQ) Why are
Page 59 and 60:
culture dishes within 1 h after pla
Page 61 and 62:
Is the coding region of the gene do
Page 63 and 64:
11. Kitts P. A., Ayres M. D., Posse
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flashBAC Manual - Oxford Expression Technologies

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