New Perspectives on the Inclusion Body Processing

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A Model Processing Route Investigated (StP)High CellDensity CultureChemicalExtraction1-step Captureof denatured L1IMAC-EBA or IMAC-HGMSas a Primary Capture Unit- Harnessing 6xHis tag of L1- Compatible with denatured proteins- Dirty feedstock can be processed- Possibility as a refolding unit
Issues in StP Route Intensification• Will it be applicable at High Cell Density ?• Other Problems:- High Viscosity- Presence of High Molecular Weight DNA- Unknown Process Compatibility with other unit operations
Page 1 and 2: New Perspe
Page 3 and 4: What Biofactories are Available?Cha
Page 5 and 6: Inclusion Body (IB) Formation in E.
Page 7 and 8: Inclusion body (IB) formation in E.
Page 9 and 10: Traditional IB Processing SchemeSol
Page 11 and 12: Approaches for Process Intensificat
Page 13 and 14: Suggested Processing Scheme (Case I
Page 15 and 16: Harnessing Chemical Extraction in I
Page 17 and 18: Operation of Expanded Bed Adsorptio
Page 19: Enabling Technology: Fusion Tag &Im
Page 23 and 24: Resolving DNA & Viscosity IssuesSpe
Page 25 and 26: Purification of L1 Using EBAEBA wit
Page 27 and 28: Binding Chemistry of L1-6x(His) to
Page 29 and 30: Processing Flowsheet in StP routeCh
Page 31 and 32: Further Improvement• Further opti
Page 33 and 34: Summary of StP-route IB ProcessingS
Page 35 and 36: Conventional IB Processing Flowshee
Page 37 and 38: Implementation of Non-solubilising
Page 39 and 40: Refolding by mimicking folding mech
Page 41 and 42: Process EconomicsC1 C2 MFermentatio
Page 43 and 44: Process Economics
Page 45 and 46: Process Economics: Pre-refolding se
Page 47 and 48: Process Economics: Effect of CPsMC1
Page 49 and 50: Overall Process Economics:[Pre-refo
Page 51 and 52: Process Economics: Column Refolding
Page 53 and 54: Conclusions• Improved understandi

New Perspectives on the Inclusion Body Processing

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