New Perspectives on the Inclusion Body Processing
New Perspectives on the Inclusion Body Processing
New Perspectives on the Inclusion Body Processing
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Inclusi<strong>on</strong> <strong>Body</strong> (IB) Formati<strong>on</strong> in E. coli (I)• What is IB? Aggregated folding intermediates somehowescaped proteolytic degradati<strong>on</strong> Must be fur<strong>the</strong>r processed to regain itsbiological activityIB• Why IB is formed? E. coli has rarely seen large, complex proteins (<strong>on</strong>ly 13% of 4300 proteins > 55 kD) Different cellular envir<strong>on</strong>ment or protein quality c<strong>on</strong>trol network not optimized foreukaryotic protein expressi<strong>on</strong> Overloading of chaper<strong>on</strong>e network or foldase (e.g. PPI, PDI) processing capability
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