New Perspectives on the Inclusion Body Processing
New Perspectives on the Inclusion Body Processing
New Perspectives on the Inclusion Body Processing
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Implementati<strong>on</strong> of N<strong>on</strong>-solubilising ChemicalExtracti<strong>on</strong> in IB <strong>Processing</strong> (PtS-route)Screening of Extracti<strong>on</strong> C<strong>on</strong>diti<strong>on</strong>1. 2-8 M urea, EDTA, Tris, pH 9 + oxidantN<strong>on</strong>-solubilising Extracti<strong>on</strong>2. Trit<strong>on</strong> X-100, urea, EDTA, Tris, pH 93. Trit<strong>on</strong> X-100, EDTA, Tris, pH 9Extracti<strong>on</strong>Diafiltrati<strong>on</strong>/Purificati<strong>on</strong>+MF-assistedRefoldingDissoluti<strong>on</strong>
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