The Production of Therapeutic Proteins in Plants: Apo AI and Insulin
The Production of Therapeutic Proteins in Plants: Apo AI and Insulin
The Production of Therapeutic Proteins in Plants: Apo AI and Insulin
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Plant Impurity AssaysPhytate (IP 6 ) is the plant’s phosphate storage molecule<strong>and</strong> requires phytase to release the phosphate.Phytate is an anti-nutrient – will b<strong>in</strong>d important m<strong>in</strong>erals<strong>in</strong> the body such as calcium, z<strong>in</strong>c <strong>and</strong> iron.Phytate is assayed by digestion with phytase thenquantitation <strong>of</strong> phosphate us<strong>in</strong>g a commercial phosphateassay reagent (Bio-Mol Green) <strong>in</strong> a microplate format(620 nm).Limit <strong>of</strong> Detection =
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