POTENTIAL BIOGAS PRODUCTION FROM FISH WASTE AND ...

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Chen Shi TRITA LWR Degree Project 12:37Fig. 10 AMPTS II setup.According to the outcomes in Tomczak-Wandzel (personalcommunication, February 2012), 40 % substrate VS based on VS contentwas the first choice in this project to promote the biogas yield due to itsshort digested time and relative high efficiency. In addition, the digestedcondition was set in the mesophilic condition (37 ± 0.5 ºC) with slowlystirring in every one minute.The most important part of this project design was to determine theproportion of mixtures in order to obtain high biogas yield. Fishintestine and by-catch contained much nitrogen source, causing low C:Nratio. It could lead to ammonia inhibition of the digestion process.Therefore, the proportion of substrates in this project was determinedbased on the wet weight of sludge in order to increase the C:N ratio,which were mainly divided into two groups, i.e. mixing with 33 % sludgeand mixing with 50 % sludge. In addition, for researching the influencedegree of fish intestine and by-catch on the AD process respectively,each group was separated into five small groups again, which contained0 %, 33 %, 50 %, 67 % and 100 % of fish intestine relative to thecontent of by-catch based on the wet weight (Table 3). The expectedresult would be obtained according to the combination of bothconsiderations.In the light of the design of the mixtures, there were 10 small groupsneeded to be analyzed to figure out the optimal outcomes. Each groupneeded 2 bottles to simulate the digestion process at least. In addition,the blank test for inoculums was also needed to be conducted tomeasure the CH 4 production from inoculums itself. The average of CH 4production from blank tests could be subtracted from it produced fromsamples in order to get the CH 4 yield from mixed substrates. However,the AMPTS II had limitation of reactors in the water bath which at most6 groups and three blank tests could be run each time. Therefore, therewere two experiments had to be performed to analyze the biogas yieldfrom 10 groups, i.e. A2, A3, A4 and B2 were in Test I and the rest were16
Potential Biogas Production from Fish Waste and Sludge1 2 1 2 334 4 5 5 66Fig. 11 The auxiliary instruments 1 weighing under 200g object, 2 weighingabove 200g object, 3 pH meter 330i, 4 oven heated till 105ºC, 5 & 6 ovenheated till 550ºC.in Test II. In addition, with a view to the wastage of inoculums duringthe measurement of the TS and VS content of samples before and afterTest I, 5 % of total inoculums from Hammarby Sjöstadsverk digesterwere mixed with the inoculums before Test II in order to reach thedemand of inoculums in Test II. The reason for choosing inoculumsfrom Hammarby Sjöstadsverk instead of Henriksdal was that its longerSRT could bring higher content of microorganisms which was favorablefor the increase of biogas production. The effect of 5 % additionalinoculums on the final result was neglected in this project, due to its lowcontent.Table 3 The composition of mixtures based on the wet weight.GroupThe proportionof sludge in themixturesSmallGroupsTheproportion ofby-catchTheproportion offish intestineTestA1 0% 67% IIA2 22% 45% IA 33%A3 33.5% 33.5% IA4 45% 22% IA5 67% 0% IIB1 0% 50% IIB2 16.5% 33.5% IB 50%B3 25% 25% IIB4 33.5% 16.5% IIB5 50% 0% II17
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