the embryo gps® dish - IVFOnline.com

The embryo GPS ® , embryo Corral ® & Universal GPS ® DishwareSpecially designed, manufactured, tested and certified for ART procedures.No more droplet collapsing, running and mixing.The benefits of the embryo GPS ® , embryo corral ® and the Universal GPS ® designer dishware are: time savings, costsavings and major benefits to the oocyte and embryo.Benefit of less time consumption:• Set-up time – specifically designedwells make set up faster and easier.• Observation time – contoured wellbottom and ‘same’ focal distancemake locating embryos quicker.• Same dish configurationsmultiply the savings.This will result in timesaving aspects for theembryologist.Benefit of possible cost reductions:• Set-up costs – specially designed dishes save in labor andtime.• Use of media – predetermined dish design allows betterplanning for overall less media use.• Less time needed for observation of embryos reducesoverall costs and time spent on this task.• All dishes are LAL and 1-cell MEA tested –meaning greater confidence and less onsitetesting. COA for each batch included witheach shipment.• Optimal for more sensitive procedures andPGD biopsies.Results in more consistent oocyteand embryo placement andsavings in use and testing.Benefits tothe oocytes andembryos:• The overall designmeans safety in handlingembryos.• No likelihood of collapsingdroplets.• Fixed volumes per well andstandardization.• Rapid identification of ocytes/embryosat a fixed focal point (GPS).• Allows better oil coverage by dish design.• Grouping of embryos in the embryo corral ®– may improve outcome.• GPS Dishware TM are sterile Gamma Radiated;LAL and 1-cell MEA tested by independenttesting company.• Bottom of dish allows better and uniform heatexchange.• New lid allows greater gas exchange and may reducelikelihood of sealing by oil and untoward alteration of pH.• A major benefit is that the embryos will spend less timeoutside the controlled environment of the incubator.• Adaptable to different culture schemes.Benefits of theUniversal GPS ® dish:• Large wells forincreased flexibility andapplications in the lab.• Concave center wellbottoms for easier and fasterembryo location andobservation.• Thinner bottom for better anduniform heat exchange.• Special lid to prevent dish sealingand subsequent increased pH.• Non-toxic, medical grade, nonpyrogenicpolysterene.• 1-cell MEA and endotoxin tested byindependent company.• FDA 510(k) Cleared and CE registered.• ISO13485:2003 ISO9001:2000.The embryo GPS ® , embryo corral ® , and Universal GPS ® dishware have been painstakingly designed to help improve safetyand make life simpler for everyone.www.IVFonline.comwww.SunIVF.com

The embryo GPS ® dish with its “GPS” location system improves security andsafety of embryos and reduces time in a ‘drop-less’ environment……No More Droplet running, collapsing, or mixingThe embryo GPS ® dish gives you structured wells and observation enhancements that allow you to save time and effortand improves safety in handling embryos.A ‘drop-less’ environment with designed wells, the ‘GPS’ location for embryos with the ‘same’ microscope focaldistance. The GPS Dishware TM line provides even and better temperature transition and distribution for a safe andconsistent workplace for embryo and other sensitive tissue culture.The ‘GPS’ location system provides you time savings and improved safety …all the time.The 8 outer wells have ‘walls’ to culture in a ‘drop-less’environment; no more droplet collapsing. All 8 wells havethe ‘GPS’ locator spot. Ease in set-up, identification,observation, handling and increased safety.Safety comes withthe ‘GPS’ system.Each embryo restssafely in each well.No more dropscollapsing or minglingduring transportation.The wells provideuniform media volumeand focal distance. The‘wells’ make oil overlaymuch easier and faster.GPS culture systemIn the GPS culture dishes each embryorests in an exact spot in each well and atthe exact same focal distance.Each embryo will be found at the exactlocation saving time and effort inlocating and observing embryos.Each batch is 1-cellMouse Embryo Assay(MEA) and LimulusAmebocyte Lysate (LAL)tested by independenttesting company. Eachbatch comes withCertificate of Analysis.The bottom: Thinner bottom forbetter heat exchange andspace for patient identification.Embryo GPS ® dish has 3large center wells forculturing, quick embryolocating and observation.New lid design allowsgreater gas exchangewith posts to preventoil-induced sealing.FDA 510(k) ClearedISO 13485:2003ISO 9001:2000GPS Dishware TM are made with testednon-toxic medical grade plasticmanufactured by Nalge NuncInternational exclusively for SunIVF.Proven safe in IVF and ART culture.The embryo GPS ® dish provides 11 exact locations (3 central wells + 8 outer wells).embryo GPS ® – The best dish for PGD cases.www.SunIVF.comwww.IVFonline.com

The embryo corral ®…THE ONLY CHOICE FOR GROUP CULTUREThe embryo corral ® is the only culture dish that allows group culture of embryos while allowing individual evaluationof each embryo. The “fence” of vertical posts in each central well prevents the embryos from passing from onequadrant to another, while still allowing exchange of culture media and embryo-derived autocrine and paracrinegrowth factors to among the embryos in the 4 quadrants of the 2 central wells, assisting embryo development.The 8 outer wells have ‘walls’ to culture in a‘drop-less’ society, media will not collapse.All 8 wells have the ‘GPS’ locator spot. Thesame ease in set-up, identification,observation and handling and safety.Each central well has 4 quadrants for groupingembryos and keeping specific identification. The‘GPS’ system makes ease in locating, observation,handling and overall safety.Safety comes with the ‘GPS’system. Each embryo restssafely in each well. No moredrops collapsing or minglingduring transportation.The wells provide uniformmedia volume and focaldistance. The ‘wells’ make oiloverlay much easier andfaster.New lid design allowsgreater gas exchangewith posts to preventoil-induced sealing.FDA 510(k) ClearedThe bottom: Thinner bottomfor better heat exchange andspace for patient identification.ISO 13485:2003ISO 9001:2000• Embryos are held in separate quadrants.• Medium, with autocrine and paracrine factors, can circulate among the quadrants.Each batch is 1-cellMouse Embryo Assay(MEA) and LimulusAmebocyte Lysate (LAL)tested by independenttesting company. Eachbatch comes withCertificate of Analysis.GPS Dishware TM are made with tested non-toxic medical grade plastic manufactured by Nalge Nunc International exclusivelyfor SunIVF. Proven safe in IVF and ART culture.The embryo corral ® dish provides 16 exact locations (2 central wells with 4 quadrants each + 8 outer wells).www.IVFonline.comwww.SunIVF.com

Introducingthe Universal GPS ® dishthe newest member of our GPS Dishware TM Line…for embryo and other sensitive tissue cultureThe Universal GPS ® dish gives you:• Large wells for increased flexibility andapplications in the lab.• Concave center well bottoms for easier and fasterembryo location and observation.• Thinner bottom for better and uniform heatexchange.• Special lid to prevent dish sealing and subsequentincreased pH.• Non-toxic, medical grade, non-pyrogenicpolystyrene.• 1-cell MEA and endotoxin tested by independentcompany.• FDA 510(k) Cleared and CE registered.• ISO13485:2003 and ISO9001:2000.All GPS Dishware TMGPS Dishware TM products are specifically designed, manufactured, tested and certified for IVF and othersensitive culture. All GPS Dishware TM products have structured micro wells eliminating collapsing andmixing droplets with concave bottoms for easier and faster cell location. The GPS Dishware TM line providesspecific innovative designed dishes to be used for specific procedures to increase safety, improve cultureperformance, and reduce time of procedure.All our dishes have self-contained wells with GPS which allows the use of defined measured amounts ofmedium and allows rapid identification of each specimen. Rapid identification means that the embryos canbe returned to their controlled environment sooner.The GPS Dishware TM design gives you safety in a ‘drop-less environment’. Its structured walls far surpassdroplets for handling safety because there is no possibility of moving collapsing droplets. Its overall designlets you use any medium and to quickly apply the oil overlay.All of our dishes give you a clearer, thinner dish bottom for better optics and more uniform temperaturecontrol.Our specially designed lid prevents dish sealing and better CO 2 exchange.We only use non-toxic, medical grade, non-pyrogenic polystyrene and all dishes pass strict 1-cell MEA,endotoxin and sterility testing by an independent company.All dishes are FDA 510(k) Cleared and CE registered, and are manufactured by our ISO13485:2003 andISO9001:2000 companies. Made in the USA.www.SunIVF.comwww.IVFonline.com

THE EMBRYO GPS ® DISH – YOUR PGD SOLUTIONThe embryo GPS ® dish is a unique and practical way to be responsible with your embryos when performingPGD procedures.• The predetermined wells in the embryoGPS ® dish significantly lower the likelihoodof mixing droplets, therefore, you’re alwaysable to identify each embryo.• The features of the embryo GPS ® dish allowyou to locate the embryo faster, making theprocedure shorter so embryos will spendless time out of the controlled environmentof the incubator.• The embryo GPS ® dishware’s speciallydesigned lid prevents the lid sealing andincreased pH. Its thinner bottom allowsmore stable and better heat exchange.• 1-cell MEA and LAL tested. FDA 510(k)Cleared and CE certified.Please call for a FREE sleeve of embryo GPS ® dishes to see the benefits for PGD biopsy procedures.Ordering/Sampling InformationProduct Description Quantity Part Numberembryo GPS ® Culture Dish The first dish designed solely for IVF. 50pk (10/sleeve) EGPS-050embryo GPS ® Culture Dish The first dish designed solely for IVF. 100pk (10/sleeve) EGPS-100embryo GPS ® Culture Dish The first dish designed solely for IVF. 500pk (10/sleeve) EGPS-500embryo corral ® Culture Dish Optimum for embryo growth and group culture. 50pk (10/sleeve) EMBC-050embryo corral ® Culture Dish Optimum for embryo growth and group culture. 100pk (10/sleeve) EMBC-100embryo corral ® Culture Dish Optimum for embryo growth and group culture. 500pk (10/sleeve) EMBC-500Universal GPS ® Culture Dish For embryo and other sensitive tissue culture. 50pk (10/sleeve) UGPS-050Universal GPS ® Culture Dish For embryo and other sensitive tissue culture. 100pk (10/sleeve) UGPS-100Universal GPS ® Culture Dish For embryo and other sensitive tissue culture. 500pk (10/sleeve) UGPS-500Enriched specially designed PGD BiopsyPGD Biopsy Media formulation. Ca & Mg free w/ sucrose and is a 20 ml LPGG-020HEPES buffered solution. 8 week min. shelf life. 50 ml LPGG-050What Our Customers Say“As of January we switched to the embryo corral ® dishes made by SunIVF. We have not had any more problems with thespreading of media drops. We are using less oil and we have noticed an increase in our clinical pregnancy rate for the firstsix months of this year compared to the same time in previous years”. Toni Di Berardino MSc, Laboratory Director, Mount SinaiHospital, Toronto, ON, Canada“Although spreading medium droplets is a phenomenon that many of us have faced thru our careers, the introduction of theembryo corral ® and the embryo GPS ® dishes from IVFonline have eliminated that problem in our laboratory. In addition tothe benefits of a fixed volume per well and rapid identification of the oocyte/embryos at a fixed focal point the dishes arevery easy to prepare and use for culture. The benefits of the group culture quadrant in the embryo corral ® dishes have beenremarkable. They are very adaptable to many culture schemes. Perhaps employing dishware specifically designed and testedfor IVF is the answer to the droplet issues”. Charlene Alouf, PhD, Laboratory Director, Crozer Chester Medical Center FertilityCenter, PA, USAwww.IVFonline.comwww.SunIVF.com

GPS Dishware TM…the future of ART and other sensitive cultureSpecific dishes designed, manufactured, tested and certified for ART procedures.Universal GPS ®Our new Universal GPS ® dish gives you:• Larger wells than the embryo GPS ® for the use of largervolumes of medium.• Each dish has structured wells eliminating collapsing dropletsand increasing safety.• The bottom of each well has our unique GPS feature – theembryos are at the center of the well, and at the same focaldistance.The 8 outer wells will each hold up to 100 μl of medium, and the2 inner wells will each hold up to 150 μl.embryo GPS ®The embryo GPS ® gives major advantages when culturing.• Each dish has structured wells eliminating collapsingdroplets.• Each well holds a defined volume of media to reduce set uptime.• The bottom of each well has our unique GPS feature – theembryos are at the center of the well, and at the same focaldistance.The 3 large center wells and 8 outer wells give you consistency.Large wells for flexibility in single or group culture. The 8 outerwells will each hold up to 50 μl of medium and the 3 inner wellswill each hold up to 100 μl. Embryo culture has become simplerand safer.embryo corral ®The embryo corral ® gives you:• An innovative design that gives you the advantages of groupculture, while maintaining the ability to monitor thedevelopment of each embryo individually.• The dish allows media and autocrine and paracrine growthfactors to move among the quadrants and thereby sharedamong the embryos.• The bottom of each well and each quadrant of the embryocorral ® well has our unique GPS feature – the embryos are atthe center of the well, and at the same focal distance.• Each dish has structured wells eliminating collapsing droplets.The 2 large inner wells will each hold up to 120 μl (30 μl/quadrant) of medium and allow the flexibility in single orgroup culture. The 8 outer wells each will hold up to 50 μl of medium, and can be used for culturing, washing orpreparation.www.IVFonline.comwww.SunIVF.com

Comparison of GPS and standard dishes for embryo culture: set-up andobservation times, and embryo developmentRieger, D.; Schimmel, T.; Cohen, J.; Cecchi, M.Introduction: Embryos are routinely cultured under oil in standard cell-culture dishes, in microdrops of medium thatcan flatten or run together. The embryos can be difficult to locate and examine at the edge of the microdrop. In the GPSdish, the medium is constrained in microwells. The bottoms of the wells are concave and parafocal to facilitate locationand examination of the embryos. The objectives of this study were to the compare the time required for preparation andembryo evaluation, and to compare the development of mouse embryos, between microdrop culture and culture in theGPS wells.Materials & Methods: On Day 0, ten standard 60 mm dishes (Nunc 150288, VWR Scientific) and ten GPS dishes(IVFonline, developed by authors TS, JC & MC) were loaded with 13 ml of oil. In each standard dish, eleven 50 μlmicrodrops of KSOM + 10 mg/ml BSA were under-laid on the surface of the dish. In each GPS dish, 50 μl of mediumwas under-laid into each of 11 wells. All dishes were held overnight at 37.3°C under 5% CO 2 in air. On Day 1, 100mouse zygotes were randomly assigned to be cultured in microdrops in the standard dishes, or in microwells of theGPS dishes. In each standard dish, 5 embryos were washed through 3 microdrops and placed in individual microdropsfor culture. In GPS dishes, 5 embryos were washed through 3 wells and cultured in individual wells. Embryodevelopment was evaluated on Days 2, 3, 4 and 5 and compared between the dish types by Chi-square analysis. Thetimes to set-up, and to evaluate each dish on each day, were recorded and compared between the dish types byKruskal-Wallis tests.Results: A significantly greater proportion of embryos cultured in GPS dishes developed to the morula stage andcompacted on Day 3, compared with embryos cultured in microdrops in standard dishes (Table 1). There were nodifferences in development on Days 2, 4 or 5. The mean times required to set-up the dishes on Day 1, and to evaluatethe embryos on Day 2, 3, 4 and 5, were all significantly less for GPS dishes than for standard dishes (Table 2).Table 1. Embryo development. Values are percentages of 50 zygotes.Development GPS Standard PCleavage on Day 2 100.0 98.0 1.000Morulae on Day 3 58.0 28.0 0.002Compacted on Day 3 92.0 78.0 0.050Blastocyst on Day 4 98.0 98.0 1.000Expanded blastocyst on Day 4 64.0 58.0 0.539Blastocyst on Day 5 98.0 96.0 0.558Expanded blastocyst on Day 5 96.0 92.0 0.400Hatched blastocyst on Day 5 2.0 2.0 1.000Table 2. Procedure times. Values are minutes/dish (mean ± sem, N = 10).Procedure GPS Standard PSet-up on Day 1 1.36 ± 0.04 1.56 ± 0.03 0.004Evaluation on Day 2 0.90 ± 0.09 1.63 ± 0.05 < 0.001Evaluation on Day 3 1.33 ± 0.11 1.90 ± 0.13 0.007Evaluation on Day 4 1.84 ± 0.12 2.58 ± 0.15 0.002Evaluation on Day 5 1.36 ± 0.06 2.34 ± 0.14 < 0.001Conclusions: Development to blastocyst by Day 5 was not different between embryos cultured in wells of the GPSdish and those cultured in microdrops in standard dishes, indicating that the GPS dishes support embryo developmentas well as do the standard dishes. The times required to set up the dishes and to evaluate the embryos were allsignificantly less for the GPS dishes than for the standard dishes. It is not clear why development on Day 3 was betterfor embryos cultured in the GPS dishes than for those cultured in the standard dishes, but may be related to the reducedtime outside the incubator. The results suggest that the use of GPS dishes for human embryo culture could reduce timeand labor costs in ART laboratories, and perhaps improve embryo development.www.IVFonline.comwww.SunIVF.com

GPS vs. Standard Microdrop Culture Comparison of Handling TimesImproves safety and reduces time in setting up, observing and handling.15Time (minutes)1050GPSMicrodropFigure 1: Comparison of the estimated handling times required for GPS orstandard microdrop culture of one dish of 5 embryos from Day 1 to Day 5,with a change to fresh medium on Day 3. The bars represent the times for:preparation of the dishes, set-up of the cultures, and dailyevaluation of the embryos. The total handling time for microdrop culture is32% more than the total time for GPS culture. (Values are based on theresults presented in Rieger et al., 2007, Proc. 14th World Congress on IVF& 3rd World Congress on IVM, Montreal, Abstract P-1202)P < 0.0013GPSMicrodropP = 0.002P < 0.00112Time per dish (min)21P = 0.004P < 0.001P = 0.007840Day 0PrepareDishesDay 1Set-upCulturesDay 2 Day 3 Day 4 Day 5Embryo Evaluation0TotalFigure 2: The times required for set-up and embryo examination in microdrops andGPS microwells.GPSMicrodrop100N.S.D.P = 0.050N.S.D.N.S.D.N.S.D.% of Zygotes806040P = 0.002N.S.D.200CleavedDay 2 Day 3 Day 4 Day 5N.S.D.Morula Compacted Blastocyst Expanded Blastocyst Expanded HatchedFigure 3: Mouse embryo development in microdrops and GPS microwells.www.SunIVF.comwww.IVFonline.com

GPS Dishware TM for IVF, ARTand other sensitive tissue culture…Our GPS Dishware TM are designed to increase your culturing effectiveness, reduce time spent and to giveyou a quality IVF and ART product.The embryo GPS ® is made to culture eggs and embryos individually with confidence. Theembryo GPS ® has 3 large wells and 8 smaller outer wells for culturing eggs or embryos.The embryo corral ® lets you culture embryos in groups and observe their developmentindividually. The embryo corral ® has 3 large wells for group culturing and 8smaller wells for egg and embryo culturing.The Universal GPS ® has large wells for flexibility in single or group culture.The Universal GPS ® has 2 large wells and 8 smaller outer wells. The wellsare larger than the embryo GPS ® .Our GPS Dishware TM products give you uniform focal distance of eachegg or embryo for ‘quicker’ observation, pipetting and handling of allspecimens in all wells. Structured wells eliminate droplet collapse andkeep them safe.Our GPS Dishware TM are CE registered, sterile, non-pyrogenic, clear, notembryo toxic, produced to strict Quality Standards and will give you anadvantage over the rest.Value features of all dishes…• medical grade plastic• mouse assay tested, one cell to 80% fully expanded blastocyst• sterility by irradiation according to ISO 11137• CE marked according to Medical Devices Directive 93/42/EEC• LAL endotoxin passed at

7 winning features of ‘the GPS Dishware TM ’1. Improves safety and security of the embryo/oocyte or specimen.2. Each self-contained well maintains the droplets integrity. No more droplet collapsing, running or mixing.3. Each well enables rapid identification of each embryo or specimen.4. Contoured bottoms of each well provide predetermined location of specimens and better heat exchange.5. GPS Dishware TM provides single or group culture with the benefits of individual tracking.6. Redesigned lid allows increased CO 2 exchange.7. Easy setup and use. Reduce time of setup, locating and observing embryos.The embryo GPS ® , embryo corral ® and the Universal GPS ®The only culture dishes you will ever need…A better choice for embryo and other sensitive tissue culture…References1 Rieger, D.; Schimmel, T.; Cohen, J.; Cecchi, M. Comparison of GPS and standard dishes for embryo culture: set-up and observation times, and embryodevelopment. 2007, Proc. 14th World Congress on IVF & 3rd World Congress on IVM, Montreal, Abstract P-1202.2 Wiley, LM, et al. 1986. Effect of potassium concentration, type of protein supplement, and embryo density on mouse preimplantation development in vitro. FertSteril. 45(1):111-9.3 Paria, BC and Dey, SK. 1990. Preimplantation embryo development in vitro: Cooperative interactions among embryos and role of growth factors. Proc Natl AcadSci. 87:4756-60.4 Canseco, RS, et al. 1992. Embryo density and medium volume effects on early murine development. J Assist Reprod & Gen. 9(5):454-7.5 Lane, M and Gardner DK. 1992. Effect of incubation volume and embryo density on the development and viability of mouse embryos in vitro. Hum Reprod.7(4):558-62.6 Gardner, DK, et al. 1994. Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum andsomatic cells: Amino acids, vitamins, and culturing embryos in groups stimulate development. Biol Reprod. 50:390-400.7 Salahuddin, SW, et al. Effects of embryo density and co-culture of unfertilized oocytes on embryonic development of in vitro fertilized mouse embryos. HumReprod. 10(9):2382-5.8 O’Neill, C. 1998. Autocrine Mediators are required to act on the embryo by the 2-cell stage to promote normal development and survivial of mouse preimplantationembryo in vitro. 58:1303-1309.9 Gil, MA, et al. 2003. Effect of volume of medium and number of oocytes during in vitro fertilization on embryo development in pigs. 60:767-776.10 de Oliveira, AT et al. 2005. Gene expression and developmental competence of bovine embryos produced in vitro under varying embryo density conditions.Theriogenology. 64(7):1559-72.11 Pickering, SJ et al. 1995. An analysis of multinucleated blastomere formation in human embryos. Hum Reprod. 10(7):1912-22.12 Balakier, H and Cadesky, K. 1997. The frequency and developmental capability of human embryos containing multinucleated blastomeres. Hum Reprod. 12(4):800-4.13 Jackson, KV, et al. 1998. Multinucleation in normally fertilized embryos is associated witha an accelerated ovulation iduction reponse and lower implantationand pregnancy rates in in vitro fertilization-embryo transfer cycles. Fert Steril. 70(1):60-6.14 Sakkas, D, et al. 1998. Early cleavage of human embryos to the two-cell stage after intracytoplasmic sperm injection as an indicator of embryo viability.13(1):182-7.15 Scott, L et al. 2000. The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. 15(11):2394-2403.16 Van Royen, E, et al. 2003. Multinucleation in cleavage stage embryos. Hum Reprod. 18(5):1062-1069.17 Hnida, C et al. 2004. Computer-controlled, multilevel, morphometric analysis of blastomere size as a biomarker of fragmentation and multinuclearity in humanembryos. 19(2):288-293.18 Paula J. Stokes, Lalantha R. Abeydeera and Henry J. Leese, 2004. Development of porcine 1embryo in vitro and in vitro: evidence fro embryo ‘cross talk’ invitro. Dev Biol. 2005 Aug 1;284:62-71.19 Alouf, C. 2006. Technology in the IVF Laboratory: Group Culture Revisited. Fertility Vol.5: 35-38.www.IVFonline.comwww.SunIVF.comsales@IVFonline.comwww.IVFonline.comUS/Canada: 1-800-720-6375Intl.: 1-519-826-5800Fax: 1-519-826-6947

Traditional Petri Dishembryo GPS ® Dish→ running droplets→ droplets mixing→ droplets flattening→ poor temperature control→ losing track of marked droplet→ difficulty locating embryos→ sealing of dish lid & increased pH ‘drop-less’ environment micro wells designed to enhance embryoculture no droplets collapsing and mixing save time and money by reducing setuptime, observation time, testing and handling better heat conservation enhance safety easily locate & observe embryos new designed lid for better gas exchange standardization of culture, uniform volumes 1-cell MEA and LAL testing by independentcompanyStandardize ART and other sensitive culture by using GPS Dishware TMEliminate these concerns and enhance safety by using GPS Dishware TM .embryo GPS ® – The best dish for PGD cases.www.IVFonline.comTel: 1-519-826-5800Tel: 1-800-720-6375Fax: 1-519-826-6947email: gps@IVFonline.comwww.SunIVF.comwww.IVFonline.comwww.SunIVF.com©2010 SunIVF S-DISHFOLD-2010-03-16