Mehdi Gasmi, PhD

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The Baculovirus SystemAutographa californica multiple nuclear polyhedrosisvirus (AcMNPV)rBac-Rep/CappA Cap Pp10 PpHReppArBac-rAAVITRAAV-vectorITRProduced inE.coliProduced inSf9 cellsSf9 cellsCulture in disposable bioreactors in serum-free conditions

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Rachel Salzman, DVM - American Society of Gene & Cell Therapy

AAV –Bac systemSf9 MCB/WCBAmplificationBaculovirus MVBsProductionBac Rep/CapBac transgeneInfection200 L disposablebioreactorsCellAmplification

Page 1 and 2: Improvements towards the industrial
Page 3 and 4: AAV vectors in the clinic‣~30% pr
Page 5 and 6: Transfection for rAAV production at
Page 7 and 8: Transfection in suspension: lack of
Page 9: Baculovirus Technology‣rAAV produ
Page 13 and 14: vg titer (vg/mL)rAAV-Bac: YieldsRes
Page 15 and 16: Comparison rAAV transfection vs. rA
Page 17 and 18: Detailed presentation of Genethon

Mehdi Gasmi, PhD

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