13.07.2015 Views

Mehdi Gasmi, PhD

Mehdi Gasmi, PhD

Mehdi Gasmi, PhD

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Transfection for rAAV production at GNTHQ PlasmidsPurified from E.coli WCBsHEK293WCBE.coli WCBE.coli WCBCell thaw/amplification to 24 CF10DMEM-2% FCSpAd helperpRep-Cap3 PlasmidTransfectionE.coli WCBCa.Phosph.pTransgeneHarvest at 72 hpost transfectionTotal ~ 25L bulk harvestConfidential

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