Progress on the Sunflower Doubled Haploid Project - National ...

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Plant regeneration of embryonic callus and ELSMedia codeS1S2S3S4S5S6S7S8S9S10Plant regeneration mediaIngredientsMS + BAP 0.5mg/l + NAA0.5mg/l+ Sucrose 20g/l +Agar 7g/lMS + BAP 0.5mg/l + NAA0.5mg/l+ Sucrose 20g/l +Agar 7g/l+ coconutwater 100ml/lMS + BAP 1.0 mg/l + NAA0.5mg/l+ Sucrose 20g/l +Agar 7g/lMS + BAP 1.0 mg/l + NAA0.5mg/l+ Sucrose 20g/l +Agar 7g/l+coconut water 100ml/lMS + BAP 0.5mg/l+ Sucrose 20g/l +Agar 7g/l+ coconut liquid 100ml/lMS + BAP 0.25mg/l + Sucrose 20g/l +Agar 7g/lMS + Sucrose 20g/l +Agar 7g/lMS + BAP 0.5 mg/l + NAA0.1mg/l+ Sucrose 20g/l +Agar 7g/lMS + BAP 0.5mg/l + IBA0.1mg/l+ Sucrose 30g/l +Agar 7g/lMS + BAP 0.5 mg/l + NAA0.1mg/l+ Sucrose 20g/l +Agar 7g/l+coconut water 100ml/lIBA‐‐‐Indole‐3‐butyric acid.24
% of green spots andsurvival calliMaterialand mediaM4‐‐‐RHA274, M5‐‐‐PeredovikFigure 2 The first sublture of anther callus: regeneration results of twoinbred lines25
Page 1 and 2: 1Xuelin Fu 1 , Richard Horsley 1 ,L
Page 4 and 5: Doubling the haploid chromosome num
Page 6 and 7: Until now, none of the techniques u
Page 9 and 10: Embryonic callus or Embryo‐Like
Page 11: Notes:1) Basic medium: MS (macro +m
Page 15 and 16: ELS produced in one anthera cluster
Page 17 and 18: Table 5 Induction results of embryo
Page 19 and 20: Anther culture of amphiploid hybrid
Page 21 and 22: For chemical inducer 2‐HNA, sunfl
Page 23: Table 7 Anther callus induction res
Page 27 and 28: 1 stsubculture ofELS on S2medium2 n
Page 29 and 30: For plant regeneration, the inbred
Page 31 and 32: Rooting of the regenerated plantlet
Page 33 and 34: + AC ‐ACShoots in rooting mediumP
Page 35 and 36: Foreign pollen inducersInterspecifi
Page 37 and 38: Future plans• Transition from ant

Progress on the Sunflower Doubled Haploid Project - National ...

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