Progress on the Sunflower Doubled Haploid Project - National ...

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Doubling the haploid chromosome numberwill produce a doubled haploid (DH).Androgenesis consists of production of plantsfrom anthers or microspores cultured in vitro.Doubled haploids have important applications inplant genetic and breeding research.4
In sunflowers, some methods such as antherculture, microspore culture and irradiation ofpollen grains have been tried by researchersto produce DHs.Anther culture is one of the most usedmethods. Cultivated sunflowers have proven tobe very recalcitrant in anther culture, especiallyfor shoot regeneration.5
Page 1 and 2: 1Xuelin Fu 1 , Richard Horsley 1 ,L
Page 6 and 7: Until now, none of the techniques u
Page 9 and 10: Embryonic callus or Embryo‐Like
Page 11: Notes:1) Basic medium: MS (macro +m
Page 15 and 16: ELS produced in one anthera cluster
Page 17 and 18: Table 5 Induction results of embryo
Page 19 and 20: Anther culture of amphiploid hybrid
Page 21 and 22: For chemical inducer 2‐HNA, sunfl
Page 23 and 24: Table 7 Anther callus induction res
Page 25 and 26: % of green spots andsurvival calliM
Page 27 and 28: 1 stsubculture ofELS on S2medium2 n
Page 29 and 30: For plant regeneration, the inbred
Page 31 and 32: Rooting of the regenerated plantlet
Page 33 and 34: + AC ‐ACShoots in rooting mediumP
Page 35 and 36: Foreign pollen inducersInterspecifi
Page 37 and 38: Future plans• Transition from ant

Progress on the Sunflower Doubled Haploid Project - National ...

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