Progress on the Sunflower Doubled Haploid Project - National ...

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GreenhypocotylRedhypocotylRedhypocotylThe hybrid seeds are germinating in culture.These three seedlings all derived from the combination:(CMS HA412HO ×HA467) F 1× H. tuberosus (Kindred South).36
Future plans• Transition from anther culture to microsporeculture.• Continue “Foreign pollen inducer” work. Detecthaploids by counting chromosome of the hybridseedlings.•Pursue “Induced mutation” work. Dr. Brent Hulkeplans to testcross M2 progenies to determinewhether or not any individual mutation line leadsto haploid progeny.• Establish true haploid lines, develop doubledhaploid lines through chromosome doubling.37
Page 1 and 2: 1Xuelin Fu 1 , Richard Horsley 1 ,L
Page 4 and 5: Doubling the haploid chromosome num
Page 6 and 7: Until now, none of the techniques u
Page 9 and 10: Embryonic callus or Embryo‐Like
Page 11: Notes:1) Basic medium: MS (macro +m
Page 15 and 16: ELS produced in one anthera cluster
Page 17 and 18: Table 5 Induction results of embryo
Page 19 and 20: Anther culture of amphiploid hybrid
Page 21 and 22: For chemical inducer 2‐HNA, sunfl
Page 23 and 24: Table 7 Anther callus induction res
Page 25 and 26: % of green spots andsurvival calliM
Page 27 and 28: 1 stsubculture ofELS on S2medium2 n
Page 29 and 30: For plant regeneration, the inbred
Page 31 and 32: Rooting of the regenerated plantlet
Page 33 and 34: + AC ‐ACShoots in rooting mediumP
Page 35: Foreign pollen inducersInterspecifi

Progress on the Sunflower Doubled Haploid Project - National ...

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