Progress on the Sunflower Doubled Haploid Project - National ...

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EmbryoidgerminationEmbryoid on regenerationmediumSmall shootelongationEmbryoid at differentdevelopmental stagesPlant regenerationprocess of amphiploidH. pumilus ×P21Normal plantlets Abnormal plantlet28
For plant regeneration, the inbred lines did notproduce shoots from callus or ELS, unlike theamphiploids.Amphiploid H. pumilus ×P21 had the most plantregeneration of the experimental materials used.More than 100 plantlets have been produced inrooting media and soils to date.29
Page 1 and 2: 1Xuelin Fu 1 , Richard Horsley 1 ,L
Page 4 and 5: Doubling the haploid chromosome num
Page 6 and 7: Until now, none of the techniques u
Page 9 and 10: Embryonic callus or Embryo‐Like
Page 11: Notes:1) Basic medium: MS (macro +m
Page 15 and 16: ELS produced in one anthera cluster
Page 17 and 18: Table 5 Induction results of embryo
Page 19 and 20: Anther culture of amphiploid hybrid
Page 21 and 22: For chemical inducer 2‐HNA, sunfl
Page 23 and 24: Table 7 Anther callus induction res
Page 25 and 26: % of green spots andsurvival calliM
Page 27: 1 stsubculture ofELS on S2medium2 n
Page 31 and 32: Rooting of the regenerated plantlet
Page 33 and 34: + AC ‐ACShoots in rooting mediumP
Page 35 and 36: Foreign pollen inducersInterspecifi
Page 37 and 38: Future plans• Transition from ant

Progress on the Sunflower Doubled Haploid Project - National ...

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