Inspection Checklist for ABSL-3 Laboratories - Select Agent Program

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Revised November 2007ReferenceBMBL: A11-aStatementNeedles and syringes or other sharp instruments are limited to use in the animalfacility when there is no alternative for such procedures as parenteral injection,blood collection, or aspiration of fluids from laboratory animals and diaphragmbottles.ResponseYes No N/ACommentsBMBL: A11-bDisposable needles must not be bent, sheared, broken, recapped, removed fromdisposable syringes, or otherwise manipulated by hand before disposal.BMBL: A11-bBMBL: A11-bUsed disposable needles must be carefully placed in puncture-resistant containersused for sharps disposal.Sharps containers should be located as close to the work site as possible.BMBL: A11-cNon-disposable sharps must be placed in a hard-walled container for transport to aprocessing area for decontamination, preferably by autoclaving.BMBL: A11-dBMBL: A11-dBroken glassware must not be handled directly; it should be removed using abrush and dustpan, tongs, or forceps.Plasticware should be substituted for glassware whenever possible.BMBL: A11-eEquipment containing sharp edges and corners should be avoided.BMBL: A12Equipment and work surfaces are routinely decontaminated with an appropriatedisinfectant after work with an infectious agent, and after any spills, splashes, orother overt contamination.BMBL: A13Animals and plants not associated with the work being performed must not bepermitted in the areas where infectious materials and/or animals are housed or aremanipulated.BMBL: A14BMBL: A15An effective integrated pest management program is required.All wastes from the animal room (including animal tissues, carcasses, andbedding) are transported from the animal room in leak-proof containers forappropriate disposal in compliance with applicable institutional, local and staterequirements.BMBL: A15BBMBL: B1Decontaminate all potentially infectious materials before disposal using an effectivemethod.Animal care staff, laboratory and routine support personnel must be provided amedical surveillance program as dictated by the risk assessment, andadministered appropriate immunizations for agents handled or potentially present,before entry into animal rooms.BMBL: B1When appropriate, a base line serum sample should be stored.BMBL: B2All procedures involving the manipulation of infectious materials, handling infectedanimals or the generations of aerosols must be conducted withinBSCs or other physical containment devices when practical.APHIS/CDC Select Agent Program ABSL-3 Inspection Checklist Page 4 of 11
Revised November 2007ReferenceBMBL: B2StatementWhen a procedure cannot be performed within a biosafety cabinet, a combinationof personal protective equipment and other containment devices must be used.Consideration should be given to the use of restraint devices and practices thatreduce the risk of exposure during animal manipulations (e.g., physical restraintdevices, chemical restraint medications, etc).ResponseYes No N/ACommentsBMBL: B3The risk of infectious aerosols from infected animals or their bedding also can bereduced if animals are housed in containment caging systems (such as solid walland bottom cages covered with filter bonnets, open cages placed in inward flowventilated enclosures, HEPA-filter isolators and caging systems, or otherequivalent primary containment systems).BMBL: B4BMBL: B4BMBL: B4BMBL: B4BMBL: B5Actively ventilated caging systems must be designed to prevent the escape ofmicroorganisms from the cage.Exhaust plenums for these systems should be sealed to prevent escape ofmicroorganisms if the ventilation system becomes static, and the exhaust must beHEPA filtered.Safety mechanisms should be in place that prevent the cages and exhaustplenums from becoming positive to the surrounding area should the exhaust fanfail.The system should also be alarmed to indicate when operational malfunctionsoccur.A method for decontaminating all infectious materials must be available within thefacility, preferably within the areas where infectious materials and/or animals arehoused or are manipulated (e.g. autoclave, chemical disinfection, or otherapproved decontamination methods). Consideration should be given to means fordecontaminating routine husbandry equipment, sensitive electronic and medicalequipment.BMBL: B5Decontaminate all potential infectious materials (including animal tissues,carcasses, contaminated bedding, unused feed, sharps, and other refuse) beforeremoval from the areas where infectious materials and/or animals are housed orare manipulated by an appropriate method. It is recommended that animalbedding and waste be decontaminated prior to manipulation and before removalfrom the areas where infectious materials and/or animals are housed or aremanipulated, preferably within the caging system.BMBL: B5Develop and implement an appropriate waste disposal program in compliance withapplicable institutional, local and state requirements. Autoclaving of content prior toincineration is recommended.BMBL: B6BMBL: B6BMBL: B6Equipment, cages, and racks should be handled in manner that minimizescontamination of other areas.Equipment must be decontaminated before repair, maintenance, or removal fromthe areas where infectious materials and/or animals are housed or aremanipulated.Spills involving infectious materials must be contained, decontaminated, andcleaned up by staff properly trained and equipped to work with infectious material.APHIS/CDC Select Agent Program ABSL-3 Inspection Checklist Page 5 of 11
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Inspection Checklist for ABSL-3 Laboratories - Select Agent Program

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