Assessment of gonad staging systems and other ... - CiteSeerX

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Plate 15. Whole developed (F4) ovaries (both fresh). Note the difference in colour. The ovary on the right isbeginning to look somewhat flaccid, indicating that this fish had spawned previously and the ovarywas becoming deplete of oocytes. Fork length of fish: 1181and 1213 mm, respectively.ABPlate 16. Whole views of (A) a developed (F4) and (B) a pre-spawning (F5a) ovary (F5 using the simplifiedmacroscopic staging system). Both ovaries were frozen after capture.HyPlate 17. Whole and sectioned views of a pre-spawning (F5a) ovary in which the oocytes have becomehydrated (Hy) prior to ovulation and spawning (F5 using the simplified macroscopic staging system).Note the size of the hydrated oocyte in comparison to the previtellogenic oocytes (arrow). Scale bar =1mm. Fork length of fish: 1063 mm.8 Fish. Res. Rep. West. Aust.2001, 136, 1-12 Insert
HyHyPlate 18. Sectioned views of the ovary in Plate 17. The left photograph shows a hydrated oocyte (Hy) with alarge clear staining oil droplet. The yolk globules have not fully coalesced to produce a uniform yolkmass. A ‘late’ stage post-ovulatory follicle is arrowed, and is shown in magnified view in the rightphotograph. Note the granulosa cell layer (bottom arrow) that has become detached from the hydratedegg in the bottom left hand corner of the right photograph. When the oocyte is released into the lumenat ovulation this follicle (comprising the granulosa and thecal cell layers) will be left behind as thepost- ovulatory follicle. Scale bars: left = 300 mm, right = 60 mm.ABPlate 19. Final maturation of the oocyte prior to spawning. (A) Migratory nucleus stage in which the nucleus(arrowed) has moved to the periphery of the oocyte prior to it breaking down. The large oil droplet isevident and some yolk globules are still intact. (B) Hydrated stage showing the fairly uniform yolkmass which stains pale pink but is translucent when viewed macroscopically. Scale bars in bothphotographs = 300 mm.Fish. Res. Rep. West. Aust. 92001, 136, 1-12 Insert
Page 1 and 2: FISHERIES RESEARCH REPORT NO. 136,
Page 3 and 4: ContentsPageAbstract ..............
Page 5 and 6: Assessment of gonad staging systems
Page 7 and 8: commerson stocks within WA waters.
Page 9 and 10: were thawed in freshwater prior to
Page 11 and 12: the lamellae the estimate of batch
Page 13 and 14: Table 2.Descriptions used to catego
Page 15 and 16: Table 5.Results of t-tests for depe
Page 17 and 18: 3.1.2 TestesS. commerson testes are
Page 19 and 20: F5b (Spawning/Running Ripe)F5c (Pos
Page 21 and 22: wrongly called F1, mainly because c
Page 23 and 24: Table 8.Simplified macroscopic stag
Page 25 and 26: 1 1a 2 3 4 5a 5c 610014 60 4 54 143
Page 27 and 28: similar data for the tropical snapp
Page 29 and 30: 5.0 AcknowledgementsThe assistance
Page 31 and 32: 7.0 AppendicesAPPENDIX A. Field sam
Page 33 and 34: F5b (Spawning)F5c (Post-spawn)F6 (S
Page 35 and 36: APPENDIX D. Fecundity analysis work
Page 37 and 38: List of PlatesABPlate 1.In situ vie
Page 39 and 40: CA*Plate 7.Whole and sectioned view
Page 41 and 42: CAPlate 9.Whole and sectioned views
Page 43: *YGPlate 12. Whole and sectioned vi
Page 47 and 48: Plate 21. Whole views of pre-spawni

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