Production Scale Kromaton Brochure - Rousselet Robatel
Production Scale Kromaton Brochure - Rousselet Robatel
Production Scale Kromaton Brochure - Rousselet Robatel
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
TECHNICAL<br />
SPECIFICATIONS<br />
FAST CENTRIFUGAL PARTITION<br />
CHROMATOGRAPH ®<br />
Rotor Volume<br />
Injection Up to 150 gr / run<br />
Flow-rate Up to 150 ml / min<br />
Rotation Speed Up to 1000 rpm<br />
Maximum pressure 900 psi<br />
Separation time 30 min to 4h<br />
Security<br />
Additionals<br />
www.kromaton.com<br />
pilot scale ® B5000<br />
<strong>Production</strong> instrument<br />
for fractionation and purification<br />
of compounds from :<br />
> Synthetic chemistry<br />
> Biological matrices<br />
> Natural products<br />
O<br />
> Biotechnology<br />
NO SOLID SUPPORT<br />
ZERO SAMPLE LOSS<br />
NO EXPENSIVE SILICA REQUIRED<br />
LOW SOLVENT CONSUMPTION<br />
EXTREMELY VERSATILE<br />
AQUEOUS-2-PHASE SYSTEMS AVAILABLE<br />
5000 ml<br />
Easy scale-up from Bench <strong>Scale</strong> FCPC ®<br />
CH CH3 Automatic door lock, explosion proof motor<br />
Solvent leak detector which stops elution and rotation<br />
Independent drive station<br />
4 ways switch valve for normal and reversed elution<br />
Cooling system<br />
All stainless steel body<br />
O<br />
N<br />
C<br />
C<br />
N<br />
CH CH3 C<br />
C<br />
Copyright 2007 <strong>Kromaton</strong> ®<br />
N<br />
N<br />
CH CH3 CH
PARAMETERS<br />
Column volume FCPC ® 200 ml FCPC ® 5000 ml<br />
Flow-rate 5 ml / min 58 ml / min<br />
rotation speed 1400 rpm 800 rpm<br />
quantity injected 1 gr 25 gr<br />
fraction analysis HPLC C18 at 220 nm<br />
APPLICATION<br />
<strong>Scale</strong> up on peptide purification from bench scale FCPC ® 200<br />
to pilot scale FCPC ® 5000<br />
mAU<br />
1500<br />
1000<br />
500<br />
0<br />
IMPURITIES<br />
0 10 20 30 40 50<br />
Minutes<br />
HPLC of crude mixture<br />
complete<br />
automatic<br />
line<br />
Source : <strong>Kromaton</strong> R&D Laboratory<br />
MAIN PEPTIDE<br />
PUMPS (SOLVENT & SAMPLE)<br />
MAIN PEPTIDE<br />
IMPURITIES<br />
IMPURITIES<br />
France<br />
9, rue alexandre fleming<br />
49066 Angers<br />
Phone : +33 241 77 41 48<br />
KROMATON@KROMATON.COM<br />
mAU<br />
1000<br />
FCPC ® chromatogram of peptide purification<br />
PC<br />
500<br />
0<br />
0 10<br />
20 30 40<br />
Minutes<br />
50 60<br />
HPLC of peptide (green zone) after FCPC ® purification<br />
IMPURITIES<br />
usa<br />
448 ignacio blvd. #480<br />
novato, ca 94949<br />
www.kromaton.com<br />
KROMATON-USA@KROMATON.COM<br />
MAIN PEPTIDE<br />
Column volume<br />
RESULTS<br />
FCPC ® 200 ml FCPC ® 5000 ml<br />
Solvent consumption 0,7 liter 19 liters<br />
Peptide collected 220 mg at 95% 5132 mg at 90%<br />
FCPC ® 5000 allows to purify peptide from synthetic mixture<br />
with low production cost and high purity recovery<br />
UV DETECTOR<br />
for your<br />
production<br />
needs<br />
FRACTION COLLECTOR<br />
Patent : fr 02791578 A1, US : 6537452, patent applic EP : 009122786 - FCPC ® is a <strong>Kromaton</strong> ® registered trademark
Change language