Sorachai Srisuma MD PhD Sorachai Srisuma MD PhD ssrisuma@rics.bwh.harvard.edu

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Protein quantification in fluids using specific antibodies. ◦ Enzyme linked-immunosorbent assay (ELISA) ◦ Radio-immunoassayimmunoassay ◦ Western blot analysis ◦ Zymography
Primary cell isolation can be successfully performed from human tissues. ◦ Epithelial cells ◦ Fibroblasts ◦ Smooth muscle cells ◦ Endothelial cells To study the proliferative, contractile, secretory, fibrogenic properties of cells following a wide range of stimuli.
Page 1: Sorachai Srisuma MD PhD Sorachai Sr
Page 6 and 7: Epithelial Basement Subepithelial a
Page 8 and 9: Resected tissue needs to be preserv
Page 11 and 12: Bronchiole -H&E staining
Page 14 and 15: Hypoxia of airway epithelial cells
Page 16 and 17: SERPINE2 (PN1) localization in mous
Page 18 and 19: It allows detection of specific mRN
Page 20: Is performed under flexible broncho
Page 23 and 24: Stained BAL cells
Page 25 and 26: Is a noninvasive method and reflect
Page 27 and 28: Indirect monitoring of lung inflamm
Page 32 and 33: FEV 1 , FEC, FEV 1 /FVC ◦ Post-br
Page 34 and 35: Airway Hyperreactivity or Airway hy
Page 36 and 37: Plethysmograph is derived from the
Page 38 and 39: The subject makes respiratory effor
Page 40 and 41: Flow signal is positive during expi
Page 42 and 43: At constant temperature, P1V1 = P2V
Page 44 and 45: Body plethysmography is particularl
Page 46 and 47: When normal human expires fully to
Page 48 and 49: Single breath N 2 washout test p.50
Page 50 and 51: When, as a result of dynamic compre

Sorachai Srisuma MD PhD Sorachai Srisuma MD PhD ssrisuma@rics.bwh.harvard.edu

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