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CSA-Journal-2016-04

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Fig. 16 Cym. ensifolium 'Noble Lotus' strain<br />

tion with similar high PGR concentrations<br />

many shoots and mycorrhizomes died off.<br />

When the leaves had reached the top of<br />

the flasks with the Hyponex medium we<br />

deflask the plants without an additional<br />

stage on a root developing medium. Roots<br />

were soon developed in the seedling pots<br />

under regular greenhouse conditions. The<br />

first flowers showed 9 years after sowing<br />

and 4 years after deflasking, which is<br />

an extraordinarily long time in the lab.<br />

Further research would be necessary to<br />

approach the results Weihong Guo is<br />

performing.<br />

C. Chang et al. (2005) mention<br />

the slow in vitro development (1-2 years<br />

before shoots initialize) of the terrestrial<br />

Jensoa cymbidiums in comparison to<br />

epiphytic cymbidiums, a fact I confirm<br />

from our lab. CHANG et al. demonstrate<br />

the typical development of an<br />

epiphytic cymbidium with Cym. dayanum<br />

RCHB.F.,1869. It is similar to Cym.<br />

hookerianum and also to Cym. wenshanense<br />

Y.S. WU & F.Y. LIU, 1990 (section<br />

Cyperorchis), a very attractive largeflowered<br />

cymbidium from southern Yunnan<br />

we cultivate and propagate at Hengduan<br />

Biotech (Figure 18 and 19). They suggest<br />

to hybridize an epiphytic cymbidium with<br />

a terrestrial (Jensoa) species to accelerate<br />

the in vitro development of the latter. With<br />

the introduction of Cym. ensifolium into<br />

the breeding of standard cymbidiums to<br />

create mini cymbidiums this has long been<br />

Fig. 17 A seedling of Cym. goeringii in flower 7 years after<br />

sowing and 4 years after deflasking.<br />

25

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