Genetic Molecular Techiniques - Part V - RNAi, Human Disease
Genetic Molecular Techiniques - Part V - RNAi, Human Disease
Genetic Molecular Techiniques - Part V - RNAi, Human Disease
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DNA Polymorphisms - variations in DNA sequence<br />
RFLPs - restriction fragment length polymorphisms<br />
SNPs - single nucleotide polymorphisms<br />
SSRs - simple sequence repeats or microsatellites<br />
Pedigree based on RFLP analysis<br />
Fig. 9-46b<br />
Experimental Detection of RFLPs<br />
WT<br />
M<br />
Fig. 9-46a<br />
How do you find the disease causing gene (cont.)?<br />
1. Comparison of gene expression from genomic region<br />
a. Northern blotting<br />
b. in situs<br />
2. Sequence candidate genes from region<br />
Example: Sickle Cell Anemia<br />
Chromosome 11<br />
collagen<br />
ribosome<br />
hemoglobin<br />
iron transporter<br />
LOC139168