determination of genetic diversity between eggplant and its wild ...

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Figure 1.5. Production and clustering of ESTs from genomic DNA. (Source: Rudd 2003) SSR is a PCR based molecular method (Staub and Serquen 1996 and Jones et al. 1997). The principle is the detection of polymorphisms resulting from different numbers of repeat units in different individuals and is observed codominantly (Powell et al. 1996, Jones et al. 1997 and Henry 1999). The level of polymorphism is very high which makes SSR an ideal marker for mapping and diversity studies, fingerprinting and population genetics (Powell et al. 1996, Jones et al. 1997, Mohan et al. 1997 and Nunome et al. 2003a). It has an easy application procedure which is basic PCR amplification of the sample and then detection of the bands (Powell et al. 1996 and Jones et al. 1997). However, the major disadvantage is related with development of SSR primers which is defined above. 10
1.4. Solanaceae Family The Solanaceae is a family in the plant kingdom and is a member of the Magnoliophyta division which is more generally referred to as angiosperms or flowering plants. The family is one of the five families of the Solanales order which is respectively a group within the 10 orders in Asterids (APG II 2003). The family includes 90 genera and estimated species number is between 3000-4000 (Knapp et al. 2004 and WEB_10 2007). The Solanaceae family members are well adapted to different environments. They show a good dispersal to a wide region in the world and even to places that have harsh conditions such as deserts (Knapp et al. 2004, WEB_10 2007 and WEB_4 2007). As a result, the Solanaceae plants can be grown in several habitats and distributed worldwide (Figure 1.6.). Figure 1.6. The dispersion of Solanaceae family members around the world. (Source: WEB_10 2007) However, the origin of diversity for most solanaceous plants is around tropical regions with accumulation around the Amazon and Andes parts of South America, thus they have a New World origin (Daunay et al. 2001, Knapp et al. 2004, WEB_10 2007 and WEB_4 2007). There are a few exceptions, though. For example, Solanum melongena (eggplant) and some of its related species are of Asia-Africa origin and are domesticated species, thus they are of Old World origin (Daunay et al. 2001, Doganlar et al. 2002a and Doganlar et al. 2002 b). 11
Page 1 and 2: DETERMINATION OF GENETIC DIVERSITY
Page 3 and 4: ACKNOWLEDGEMENTS I want to present
Page 5 and 6: ÖZET PATLICAN VE YABAN TÜRLER ARA
Page 7 and 8: 3.1.2. Analysis and results of the
Page 9 and 10: Figure 3.1. An example of AFLP stud
Page 11 and 12: 1.1. Diversity and Systematics CHAP
Page 13 and 14: are drawn (Li 1997 and Raven et al.
Page 15 and 16: y DNA-based methods and recombinant
Page 17 and 18: 1.2.1.2. SSR Figure 1.3. The protoc
Page 19: cDNA, all of which in fact were syn
Page 23 and 24: Figure 1.7. An example of a phyloge
Page 25 and 26: 1998, Daunay et al. 1999 and Kashya
Page 27 and 28: and Chavan 1998 and Kashyap et al.
Page 29 and 30: Collonnier et al. 2001). Chlorine,
Page 31 and 32: Figure 1.11. Plant species used in
Page 33 and 34: Figure 1.12. Phylogenetic classific
Page 35 and 36: Gottlieb 1995 and Kaur et al. 2004)
Page 37 and 38: Jones et al. 1997). Digestion point
Page 39 and 40: informative for both plants and ver
Page 41 and 42: Table 2.1. Turkish eggplants charac
Page 43 and 44: Table 2.2. Eggplant and its wild re
Page 45 and 46: 2.1.2.Sample DNAs 2.1.2.1. Extracti
Page 47 and 48: Table 2.5. Wild Eggplants Nanodrop
Page 49 and 50: Other modifications in this step we
Page 51 and 52: 41 Table 2.7. Repeat motifs and seq
Page 53 and 54: 3.1. Results CHAPTER 3 RESULTS AND
Page 55 and 56: Figure 3.1. An example of AFLP stud
Page 57 and 58: 47 Figure 3.3. Dendrogram showing c
Page 59 and 60: 49 8 16 18 22 23 33 37 40 42 43 44
Page 61 and 62: and Levin et al. 2006). Intraspecie
Page 63 and 64: Table 4.1. Repeat motifs, numbers o
Page 65 and 66: 55 Table 4.2. SSR primers repeat mo
Page 67 and 68: 57 SGN EST Identifier Number of Uni
Page 69 and 70: After design of the primers and the
Page 71 and 72:
CA, USA). 1:5, 1:10, 1:20, and 1:30
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63 Figure.4.4. Dendrogram showing s
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65 Dim-2 0.40 0.20 -0.01 -0.21 8 4
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4.2. Discussion According to the st
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CHAPTER 5 CONCLUSION AND FUTURE PER
Page 81 and 82:
REFERENCES Bremer, B., Bremer, K.,
Page 83 and 84:
Karihaloo, J.L., Gottlieb, L.D., 19
Page 85 and 86:
Raven, P.H., Evert, R.F., Eichhorn,
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