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Effect of colemanite ore as boron fertilizer on growth and yield of two wheat (Triticum aestivum L.) genotypes under field condition

Abstract Wheat (Triticum aestivum L.) is the most important cereal crop in the world. The deficiency of boron (B) in soil is widespread global problem and affects crop yields. Mineral colemanite is a cheap and slow release source of B. In this dual factor field experiment we evaluated the effectiveness of colemanite ore as B fertilizer by studying its effect on growth and grain yield of two wheat genotypes. Factor A comprised five B levels (0, 1.5, 2.0, 3.0 and 4.0 kg B ha-1) in the shape of colemanite while factor B included two wheat genotypes TD-1 and TJ-83. The maximum plant height, number of tillers per plant, number of grains per ear head and seed index in both genotypes TJ-83 and TD-1 was observed at 4 kg B ha-1. The highest grain yield (4.2 tons ha-1) of the crop was also at the same B level (4 kg B ha-1). The application of colemanite increased 30% grain yield in compare to control. Although B application increased the yield at all levels but difference between 3 and 4 kg B ha-1 was not significant in both genotypes. The B content in wheat grain also augmented and B in soil after crop harvest was also high (0.9 mg kg-1) in B applied plots in compare to control plots (0.4 mg kg-1). This means subsequent crop will also get positive residual effect of colemanite. The results of the study showed that colemanite can be used as a B source for wheat crop.

Abstract
Wheat (Triticum aestivum L.) is the most important cereal crop in the world. The deficiency of boron (B) in soil is widespread global problem and affects crop yields. Mineral colemanite is a cheap and slow release source of B. In this dual factor field experiment we evaluated the effectiveness of colemanite ore as B fertilizer by studying its effect on growth and grain yield of two wheat genotypes. Factor A comprised five B levels (0, 1.5, 2.0, 3.0 and 4.0 kg B ha-1) in the shape of colemanite while factor B included two wheat genotypes TD-1 and TJ-83. The maximum plant height, number of tillers per plant, number of grains per ear head and seed index in both genotypes TJ-83 and TD-1 was observed at 4 kg B ha-1. The highest grain yield (4.2 tons ha-1) of the crop was also at the same B level (4 kg B ha-1). The application of colemanite increased 30% grain yield in compare to control. Although B application increased the yield at all levels but difference between 3 and 4 kg B ha-1 was not significant in both genotypes. The B content in wheat grain also augmented and B in soil after crop harvest was also high (0.9 mg kg-1) in B applied plots in compare to control plots (0.4 mg kg-1). This means subsequent crop will also get positive residual effect of colemanite. The results of the study showed that colemanite can be used as a B source for wheat crop.

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Int. J. Biosci. 2016<br />

The maximum straw <strong>yield</strong> w<str<strong>on</strong>g>as</str<strong>on</strong>g> recorded from the<br />

plots which received 4 kg B ha -1 . The plants from<br />

c<strong>on</strong>trol plots had minimum straw <strong>yield</strong>. In terms <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

varietal performance, straw <strong>yield</strong> obtained from TJ-<br />

83 w<str<strong>on</strong>g>as</str<strong>on</strong>g> m<str<strong>on</strong>g>ore</str<strong>on</strong>g> than TD1. Colemanite applicati<strong>on</strong><br />

significantly affected B c<strong>on</strong>centrati<strong>on</strong> in <strong>wheat</strong> straw<br />

<strong>and</strong> grain (Table 4). Laboratory analysis <str<strong>on</strong>g>of</str<strong>on</strong>g> plant<br />

samples showed that the highest B c<strong>on</strong>centrati<strong>on</strong> w<str<strong>on</strong>g>as</str<strong>on</strong>g><br />

found in the 4 kg B ha -1 plots compared to the other<br />

treatments. This same trend <str<strong>on</strong>g>of</str<strong>on</strong>g> plant B c<strong>on</strong>tent w<str<strong>on</strong>g>as</str<strong>on</strong>g><br />

observed in both <strong>genotypes</strong>.<br />

Table 3. Bor<strong>on</strong> in soil <strong>and</strong> plant after harvest.<br />

Treatment<br />

(kg B ha -1 )<br />

Soil Depth<br />

0-20 cm<br />

Soil Depth<br />

20-40 cm<br />

B c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> Straw<br />

(mg kg -1 )<br />

B c<strong>on</strong>tent <str<strong>on</strong>g>of</str<strong>on</strong>g> Grain<br />

(mg kg -1 )<br />

C<strong>on</strong>trol (0.0) 0.462 E 0.3052 E 5.0 C 3.0 B<br />

1.5 0.5400 D 0.3318 C 5.5 C 3.96 B<br />

2.0 0.8070 C 0.5352 B 6.6 B 4.66 AB<br />

3.0 0.9113 B 0.7352 A 7.8 A 5.66 A<br />

4.0 0.962 A 0.767 A 8.5 A 5.0 A<br />

SE 0.0400 0.0219 0.5321 0.4342<br />

LSD 0.0840 0.0460 0.8532 0.8340<br />

Wheat Genotypes<br />

TD1 0.8013 A 0.5445 B 7.2 5.5<br />

TJ83 0.7510 A 0.6091 A 6.9 4.7<br />

SE 0.0253 0.0138 0.4932 0.4432<br />

LSD 0.0531 0.0291 0.8124 0.7853<br />

The values with same letter within columns are not significantly different at p=0.05.<br />

As the B improved all <strong>growth</strong> parameters <str<strong>on</strong>g>of</str<strong>on</strong>g> the <strong>wheat</strong><br />

crop so ultimately the grain <strong>yield</strong> also significantly<br />

incre<str<strong>on</strong>g>as</str<strong>on</strong>g>ed at all B levels in compare to c<strong>on</strong>trol (Table<br />

2). The mean data showed significant differences<br />

am<strong>on</strong>g B levels in varieties <strong>and</strong> their interacti<strong>on</strong>. The<br />

maximum per hectare grain <strong>yield</strong> (4017.3 <strong>and</strong> 4230.0<br />

kg ha -1 ) w<str<strong>on</strong>g>as</str<strong>on</strong>g> recorded from the plots which were<br />

fertilized with 3 <strong>and</strong> 4 kg B ha -1 , respectively.<br />

Incre<str<strong>on</strong>g>as</str<strong>on</strong>g>ing B levels from 1.5 to 4.0 kg ha -1 incre<str<strong>on</strong>g>as</str<strong>on</strong>g>ed<br />

grain <strong>yield</strong> gradually minimum <strong>yield</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> grain w<str<strong>on</strong>g>as</str<strong>on</strong>g><br />

recorded in c<strong>on</strong>trol plots. Although both <strong>genotypes</strong><br />

gave positive resp<strong>on</strong>se to B applicati<strong>on</strong> but when<br />

these were compared them than TD-1 had performed<br />

better than TJ-83. The results <str<strong>on</strong>g>of</str<strong>on</strong>g> our <strong>field</strong> experiment<br />

have shown that <str<strong>on</strong>g>colemanite</str<strong>on</strong>g> applicati<strong>on</strong> upto 4.0 kg<br />

ha -1 had incre<str<strong>on</strong>g>as</str<strong>on</strong>g>ed 30% grain <strong>yield</strong> over the c<strong>on</strong>trol.<br />

Rehman (2012) reported that B applicati<strong>on</strong> w<str<strong>on</strong>g>as</str<strong>on</strong>g> main<br />

c<strong>on</strong>tributor <str<strong>on</strong>g>of</str<strong>on</strong>g> total grains m<str<strong>on</strong>g>as</str<strong>on</strong>g>s by improving the<br />

spike length <strong>and</strong> number <str<strong>on</strong>g>of</str<strong>on</strong>g> grains, number <str<strong>on</strong>g>of</str<strong>on</strong>g><br />

spikelets, 1000-grain weight <strong>and</strong> grain <strong>yield</strong>.<br />

Tahir et al. (2009) observed the resp<strong>on</strong>se <str<strong>on</strong>g>of</str<strong>on</strong>g> <strong>wheat</strong><br />

<strong>yield</strong> to applicati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> B at various growing stages.<br />

They observed that grains number per spike,<br />

thous<strong>and</strong> grain weight <strong>and</strong> grain <strong>yield</strong> w<str<strong>on</strong>g>as</str<strong>on</strong>g><br />

significantly enhanced where the applicati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> B w<str<strong>on</strong>g>as</str<strong>on</strong>g><br />

applied. Wrobel (2009) studied impacts <str<strong>on</strong>g>of</str<strong>on</strong>g> the foliar<br />

B applicati<strong>on</strong> to grown <strong>wheat</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> spring <strong>on</strong> s<strong>and</strong>y soil.<br />

The availability <str<strong>on</strong>g>of</str<strong>on</strong>g> B enhanced the grain m<str<strong>on</strong>g>as</str<strong>on</strong>g>s, straw<br />

<strong>yield</strong> <strong>and</strong> enhancing the <strong>yield</strong>. Wheat producti<strong>on</strong><br />

incre<str<strong>on</strong>g>as</str<strong>on</strong>g>ed significantly may be due to the re<str<strong>on</strong>g>as</str<strong>on</strong>g><strong>on</strong> that<br />

the applicati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> B enhances the pollen tube<br />

germinati<strong>on</strong> <strong>and</strong> grain setting at booting stage <strong>and</strong><br />

the availability <str<strong>on</strong>g>of</str<strong>on</strong>g> B through <str<strong>on</strong>g>colemanite</str<strong>on</strong>g> at this stage<br />

in the anthers for successful fertilizati<strong>on</strong> w<str<strong>on</strong>g>as</str<strong>on</strong>g> met so<br />

the grain <strong>yield</strong> boosted. These inferences are in<br />

accordance with the Alam et al. (2000) who reported<br />

that grain <strong>yield</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> <strong>wheat</strong> incre<str<strong>on</strong>g>as</str<strong>on</strong>g>ed due to the<br />

applicati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> B at different <strong>growth</strong> stages.<br />

87 G<strong>and</strong>ahi et al.

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