Oxidative And Nitrosative Modulators In Neuroinflammation
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OXIDATIVEANDNITROSATIVEMODULATORSINNEUROINFLAMMATION<br />
ConcentrationofadvancedproteinoxidationproductsTheconcentrationofAOPPwasdeterminedin<br />
plasma,haemolysatesandcerebrospinalfluidsusingH2O,KIandaceticacidbyspectrophotometricmethod<br />
at340nm (Witko-Sarsatetal1996).Thevaluesobtainedwereexpressedasµmolperliter(plasmaand<br />
cerebrospinalfluid),orgHb(hemolysate).<br />
ConcentrationofsulfhydrylgroupsThetotalamountof(proteinandnon-protein)SHgroupswas<br />
determinedinplasmaandliver,whileinthehemolysatestheconcentrationofGSHwasdeterminedusing<br />
thespectrophotometricmethodusingDTNB(SedlakandLindsay,1968).Thevaluesobtainedwere<br />
expressedasµmolperliter(plasmaandcerebrospinalfluid),orgHb(hemolysate).<br />
ActivityofsuperoxidedizmutaseSODactivitywasmeasuredbythemethodofMinamiandYoshikawa<br />
(1979),whichisbasedontheinhibitionofautoxidationofpirogalol.Asuperoxideanionformedby<br />
autoxidationofpyrogalolformsacoloredcompoundwithNBT.AstheO2-Purifier,SODinhibitsthis<br />
reaction,whereoneunitofenzymaticactivityrepresents50% inhibition.Thevaluesobtainedare<br />
expressedasUperliter(plasmaandcerebrospinalfluid),orgHb(hemolyzate).<br />
RadiologicalfindingTheCNStissuewasexaminedwithamagneticresonancewith1.5T(Avanto,<br />
Siemens,Erlangen,Germany).TheMRprotocolincludedthefolowingconventionalspinsequences:axial<br />
T1-weighted(VR)=500miliseconds,EVA=78miliseconds,numberofexcitations(BE)=2)and<br />
T2-weighted(VR=4700miliseconds,EV=93miliseconds,BE=2)withacross-sectionthicknessof5mm,<br />
andaspacebetweenthecross-sectionof0.5mm andapixelsizeof0.9x0.9mm.Anintravenously<br />
administeredGd-ContrastAgent(Gadovist,Schering,Berlin,Germany)atadoseof0.1mmol/kgbody<br />
weight.Thenumberand/orvolumeofhyperintensechangesseenonT2sequencesaswelasthevolume<br />
ofGdbindingchangesseenonT1sequencesweretakenforanalysispurposes.AlMRfindingswere<br />
interpretedbyaneuroradiologistwhodidnothaveaninsightintoaclinicalfinding,noradiagnostic<br />
classificationofpatients.<strong>In</strong>relationtothenumberofdescribedchanges,divisionwasmadewithinboth<br />
groupsofpatientstothosewithlowerandthosewithagreaternumberofradiologicalchangesthanthe<br />
meanwithineachgroup.
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