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recomLine EBV IgG [Avidity] [IgA] recomLine EBV IgM ... - Mikrogen
recomLine EBV IgG [Avidity] [IgA] recomLine EBV IgM ... - Mikrogen
recomLine EBV IgG [Avidity] [IgA] recomLine EBV IgM ... - Mikrogen
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Test Principle and Procedure<br />
Typical course of an <strong>EBV</strong> infection<br />
1. Sample<br />
2. Sample<br />
1 Week<br />
3. Sample<br />
2 Weeks<br />
4. Sample<br />
4 Weeks<br />
5. Sample<br />
8 Weeks<br />
6. Sample<br />
14 Weeks<br />
7. Sample<br />
11 Months<br />
E E E<br />
<strong>IgG</strong><br />
Avid.<br />
<strong>IgM</strong><br />
<strong>IgG</strong><br />
Avid.<br />
<strong>IgM</strong><br />
<strong>IgG</strong><br />
Avid.<br />
<strong>IgM</strong><br />
<strong>IgG</strong><br />
Avid.<br />
<strong>IgM</strong><br />
<strong>IgG</strong><br />
Avid.<br />
<strong>IgM</strong><br />
<strong>IgG</strong><br />
Avid.<br />
<strong>IgM</strong><br />
<strong>IgG</strong><br />
Avid.<br />
<strong>IgM</strong><br />
Cutoff<br />
EBNA-1<br />
p18<br />
p23<br />
ZEBRA<br />
BZLF1<br />
p138<br />
p54<br />
+ - - - +++ + ++<br />
+ - ++ +/- +++<br />
+ - - ++ +++ ++ ++<br />
+ - - ++ +++ ++ ++<br />
+ - - ++ ++ ++ ++<br />
+ - + - ++<br />
+ - + ++ ++ ++ ++<br />
+ - + - ++<br />
+ - ++ ++ ++ ++ +<br />
+ - - - +<br />
+ + ++ ++ + + -<br />
+ - - - -<br />
Article-No Storage and Shelf Life<br />
4572 <strong>recomLine</strong> <strong>EBV</strong> <strong>IgG</strong> [<strong>Avidity</strong>]* [<strong>IgA</strong>]*<br />
Reagents for 20 determinations<br />
4573 <strong>recomLine</strong> <strong>EBV</strong> <strong>IgM</strong><br />
Reagents for 20 determinations<br />
10076 Line - anti-Human <strong>IgA</strong> conjugate, 500 µl<br />
11010 Blot, Line - avidity reagent<br />
Reagent for 25 avidity determinations<br />
1 st Incubation A test strip loaded with <strong>EBV</strong> antigens is incubated with<br />
diluted serum or plasma in a dish for 1 hour.<br />
+ - ++ +/- +++<br />
+ - + - +++<br />
positive<br />
p23 interm.<br />
positive<br />
positive<br />
p23 high<br />
p18 low<br />
positive<br />
positive<br />
p23 high<br />
p18 low<br />
positive<br />
positive<br />
p23 high<br />
p18 high<br />
negative<br />
*[] optional available as additional reagent<br />
wash 3 times<br />
2 nd Incubation Peroxidase conjugated anti-human antibodies (<strong>IgG</strong>, <strong>IgA</strong><br />
or <strong>IgM</strong> specific) are added. Incubate for 45 minutes.<br />
wash 3 times<br />
Color reaction 5 - 10 minutes after addition of the coloring solution,<br />
insoluble colored bands develop at the sites on the test<br />
strips occupied by antibodies.<br />
positive<br />
---<br />
positive<br />
positive<br />
p23 low<br />
positive<br />
positive<br />
p23 interm.<br />
positive<br />
acute<br />
infection<br />
acute<br />
infection<br />
acute<br />
infection<br />
acute<br />
infection<br />
recent<br />
infection<br />
recent<br />
infection<br />
past<br />
infection<br />
BZLF1 <strong>IgG</strong> and ZEBRA <strong>IgM</strong> appear<br />
as early markers beside the EA antigens<br />
(<strong>IgG</strong> and <strong>IgM</strong>)<br />
p18 <strong>IgG</strong> appears as late marker in<br />
the 8th week (sample 5)<br />
<strong>Avidity</strong> of p23 <strong>IgG</strong> rises during<br />
approx. 7 weeks (sample 2-5)<br />
EBNA-1 <strong>IgG</strong> appears as late marker<br />
after 11 months (sample 7)<br />
At +2°C - +8°C<br />
18 months from the date of production<br />
MIKROGEN GmbH | Floriansbogen 2-4 | 82061 Neuried | Germany | Tel.: +49 89 54801-0 | Fax: +49 89 54801-100<br />
Internet: www.mikrogen.de | eMail: mikrogen@mikrogen.de<br />
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pirlebe008n
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