Table 1I.Results otfeeding Animal Ni 229 II .pakai- .n d nymphon hrte rem State led r tNlll 30 243 resulialil s 23l utcecpliblc i, 24! siiscejiiiptie .47 251, sis1epihlc " 43 Coniitrtl ra i t i ( "o rtnd r,,hb it . Iur lies dislphiccl. iull litcks liM had moulted, to pick tip7.parva.The fail ore of 2/3 of the susceptibles to become infected also cast serious doubts on the infectivity of the ticks, and thus detracted from the apparently favourable outcome with the tick-resist ant cattle. There is,however, tit) doubt that we succceded in rendering the group I cattle resistant, which confirrins our findiniiethe ill li~ild that 11.illI('it s dlCchp -CistanoCe tituile sIsssl" uid less taikCtikh (h,tid ti h\ ,t te1t)1 0,1IAN Ill IIAI ION AND (tARA(IFRISA I ION (11- ANI( d NS I-t( )M SAI.IVARfY i.ANt)S, R t 'ROII(VI IVI- SYSI I M. (itt ANt) tIAMt)IYNIt'lI it PAR IIAtH F I) I I N,1I.I V I (KS 0 - R/II'I( TI.I ,/S. ,'#'ll It Tlhe studies undertaken involved immunis,tion of animals with crude antigen extracts derived from unfed, partially fed or fully fed ticks. Initial information on protein components oh R?. aippendicrlatis organs producing the antigens responsible for the resistance response against ticks is described in this summnary,. Soditit doidecyl sulfate-polyacrylamide g,:l electrophorcsis SI)S-PA( I-) :igure 0) relsolsedIt las,t 52 proIteit bands detected by C(omssie Blue staining in the salivary glands, 42 in the reproductive system, 40 in the gut and 41 inthe haemolymph. Itneah of these organs there were several unenumerated indistinct hands. There also appears to be a number of protein bands shared by all 4 tick tissue organs. Although these protein hands may be of similar molecular weight, they need not necessarily ave the same primary structure and fuiction. Immune-precipitation sttidies were performed using antiserum from a rabbit previously immiunised with unfed tick homogenate and with lyophilised homogenate of fully fed female ticks. (Ticks feeding on this rabbit showed reduced reproductive potential). The tick antigen extract used was radiolabeLed with IsS methionine and 11S cystein. The selected anti-tick serum immune-precipitated (in the presence of Stap/hi'loco'cus aureao) 9-10 radiolabelled proteins (Figure 7) as analveld by SI)S-l'A(.L The molecular weights of these proteins were 180,000; 160,000; 140,000; 130,000; 98,000; 92,000; 88,000; 85,000; and 82,000 daltons. The pattern of the specifically immune precipitated proteins was remarkably similar between the tick tissie antigen extracts (Figure 7). It is hypothesised from these results that the antigen causing the reduced reproductive resI onse in ticks may be u 11 l ,tSCe'ibt li' 'attle'1odltick-mmi \1calI't 4I1ig) ( litijil restil, i . no icci n IolaS lo , 7 n., ilctuil; [I' .1-- 7 - ? . Crlr, I 10ti1, uclll t'01 o i 'luc y 0 I fu i t % nI n1c cll 'iin sercm-soin% ci X.S Io Ilnlslt l , no I i c- 'l l l hd t I '0 [Lis2l 30 2 1 o', 75 I.d ) Iillpli one o lthe recognized antigetis. Detailed studies ale bei,-g carried out, characterising and isolating sufficien, quantitie, ol native or subunit proteins for further i',imunisatioit trials, using the gut as the selected otgait. 160,000 wthwi, ,92 500WM 67,0000 39000 25,800, 14,500- e .W1 '15 A B C D " i1 ! Figure 6. Electrophoretic anallsis of tick priteins derived from salt vary glands (A), reproductive system (1), gut (C), and haemolymph (D) of I-day fed female R. appendiculatus. Molecular weight marker proteins In dallons areIndicated in themargin on tlie left of the gel: RNA plymerase, 160,000 and 39,000; phoslphorylase 'a'92,000; bovine serum albumin, 67,000; chymotrypslonogen, 25,800 and lysozyme 14,500. ., V
160,000. A B C D E F G H 92,500 -._ - . . 67,000 . 25,80o 14,500- Iigur, 7. An ;auhoradiogruph iofiniun,, precipi ated radhlhlabheh, lick protdn, obtained from salivary glands (11),reproductive system (I)), gut IF) and hrn, i l iph %, I IhI of 5-dj "sfed fr mali I ,/ljit dlizuhil se l-ge 6). ILanes A, C, F and G are 'ontrol precipitalions Mille Lanes F'.11, It, re l i d i), Ii 'pe'ifiril. imune precipaeit'd polv pe es fromt the crrespfiudifig tiSSeJ,% eFigure 6. Molecular %eight markers a%in Figure 6 31
- Page 1 and 2: -A 11c \A- N .
- Page 3 and 4: 1986 Retirement class (April 1986)
- Page 5 and 6: Contents Governing Board Donors For
- Page 7 and 8: Crop Pests 13iononi ics and applicd
- Page 9 and 10: Crop Pests IIIONOMIC'S AN!) APIt.It
- Page 11 and 12: C'op PeIs numberofmalesmated;themat
- Page 13 and 14: Crop IP'ex Evaluation of Additional
- Page 15 and 16: Crop IP'nt being susceptible and th
- Page 17 and 18: canopy while in others peduincies w
- Page 19 and 20: Crop Pests temperature of 25°C. Ob
- Page 21 and 22: the population studies of the pest
- Page 23 and 24: Crop Pests Table I Primary u cs ol
- Page 25 and 26: _Research Programme WLivestock Tick
- Page 27 and 28: showed a capacity to control their
- Page 29 and 30: RESISTANCE INDUCED BY LABORATORY AN
- Page 31: ate and market value. The number of
- Page 35 and 36: Medical Vectors --- Research Progra
- Page 37 and 38: Table 3 Inoculation olf io e ", ih
- Page 39 and 40: Ctlrtlaiintingtlir ,us ,Crc eattfmi
- Page 41 and 42: Ase 'Ijucse anid I Iypflritsoijasis
- Page 43 and 44: Population data analysis R.D. Drawf
- Page 45 and 46: Tvetve COINI UNITY I'AR''C:ItA It'I
- Page 47 and 48: lsetse THE COINCIDENCE OF VIRt'S-.I
- Page 49 and 50: Tsts.e (BUT), 14 stocks produced po
- Page 51 and 52: against TAME. The individual fracti
- Page 53 and 54: aised (Eleventh Annual Report, 1983
- Page 55 and 56: Chemistry andi Bioassaiy Svnopsis o
- Page 57 and 58: Chemistry and BIoa%vin Pheromone an
- Page 59 and 60: It is an important finding that the
- Page 61 and 62: Chemistry and Bioassay needles (m.p
- Page 63 and 64: 90- +-90 70- 70 5 50 30- 30 1 10 40
- Page 65 and 66: Chenuirv and Ili..st "('-tnr tl; *t
- Page 67 and 68: ;Histology and Fine Structure Resea
- Page 69 and 70: N7c5 etk un n t im te Itll. (1 li s
- Page 71 and 72: FHII(ROPtYSIOI.OGICA. ANI ttttAVI(U
- Page 73 and 74: Fle'I, I IIlt,: I , dco . IlInIII 1
- Page 75 and 76: Sen.ory'chyiiology Tahble 2. Chilop
- Page 77 and 78: J he available data sho, that the E
- Page 79 and 80: Outreach and 'I'raining Outreach an
- Page 81 and 82: Outreachand Training more structur-
- Page 83 and 84:
Outreach arid Iramiiii senior IC IP
- Page 85 and 86:
_a Computer A('COMPLISHiMEN'I S 1.
- Page 87 and 88:
are being used for data processing.
- Page 89 and 90:
Communication and Information Divis
- Page 91 and 92:
* The goal must be to reach the ful
- Page 93 and 94:
Management The management and admin
- Page 95 and 96:
1985 Publications Alghalh. A M , In
- Page 97 and 98:
1985 Personnel ()FFI('i()I I I MlRI
- Page 99 and 100:
Mr. A.A. I atif.Phi.). tuid,,it Mr.
Change language